Incorporation of NS1 and prM/M are important to confer effective protection of adenovirus-vectored Zika virus vaccine carrying E protein

被引：29

作者：

Liu, Xinglong ^{[1
,2
]}

Qu, Linbing ^{[1
]}

Ye, Xianmiao ^{[1
,3
]}

Yi, Changhua ^{[1
]}

Zheng, Xuehua ^{[1
]}

Hao, Mingli ^{[2
]}

Su, Wan ^{[2
]}

Yao, Zhipeng ^{[4
]}

Chen, Peihai ^{[4
]}

Zhang, Shengnan ^{[1
]}

Feng, Yupeng ^{[1
]}

Wang, Qian ^{[1
,3
]}

Yan, Qihong ^{[1
,3
]}

Li, Pingchao ^{[1
]}

Li, Heying ^{[1
]}

Li, Feng ^{[5
]}

Pan, Weiqi ^{[5
]}

Niu, Xuefeng ^{[5
]}

Xu, Ruian ^{[2
]}

Feng, Liqiang ^{[1
]}

Chen, Ling ^{[1
,2
,5
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Labs Resp Dis, Guangzhou, Guangdong, Peoples R China

[2] Huaqiao Univ, Sch Biomed Sci, Quanzhou, Peoples R China

[3] Univ Chinese Acad Sci, Beijing, Peoples R China

[4] Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Anhui, Peoples R China

[5] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Guangzhou, Guangdong, Peoples R China

来源：

NPJ VACCINES | 2018年 / 3卷

基金：

中国国家自然科学基金;

关键词：

DENGUE VIRUS; DNA VACCINE; JAPANESE ENCEPHALITIS; T-CELLS; ANTIBODIES; EFFICACY; MICE; IMMUNOGENICITY; REPLICATION; CANDIDATES;

D O I：

10.1038/s41541-018-0072-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Current design of Zika virus (ZIKV) vaccine mainly considered envelope (E) as the major target antigen. Non-structural protein NS1 was seldom considered. Herein, we generated three adenovirus-vectored vaccines carrying E (Ad2-E), or premembrane/membrane (prM/M) with E (Ad2-prME), or NS1 in addition to prM/M with E (Ad2-prME-NS1). Ad2-prME induced higher neutralizing antibody response to ZIKV than Ad2-E, suggesting prM/M is important for the folding of immunogenic E. Most intriguingly, Ad2-prME-NS1 elicited the best viral inhibition when the immune sera were added to ZIKV-infected cells. In ZIKV-challenged neonatal mice born to maternally immunized dams, Ad2-prME-NS1 conferred the best protection in preventing weight loss, neurological disorders, and viral replication. Ad2-prME also conferred significant protection but was less effective than Ad2-prME-NS1, whereas Ad2-E only alleviated neurological symptoms but did not inhibit viral replication. Our study suggested that NS1 should be considered in the design of ZIKV vaccine in addition to prM/M and E.

引用

页数：8

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