Differentiation of embryonic stem cells into retinal neurons

被引:91
作者
Zhao, X [1 ]
Liu, JN [1 ]
Ahmad, I [1 ]
机构
[1] Univ Nebraska, Med Ctr, Dept Ophthalmol, Omaha, NE 68198 USA
关键词
ES cells; stem cells; retina; eye; photoreceptors; differentiation; bipolar cells;
D O I
10.1016/S0006-291X(02)02126-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mouse embryonic stem (ES) cells are continuous cell lines derived from the inner mass of blastocysts. Neural progenitors derived from these cells serve as an excellent model for controlled neural differentiation and as such have tremendous potential to understand and treat neurodegenerative diseases. Here, we demonstrate that ES cell-derived neural progenitors express regulatory factors needed for retinal differentiation and that in response to epigenetic cues a subset of them differentiate along photoreceptor lineage. During the differentiation, they activate photoreceptor regulatory genes, suggesting that ES cell-derived neural progenitors recruit mechanisms normally used for photoreceptor differentiation in vivo. These observations suggest that ES cells can serve as an excellent model for understanding mechanisms that regulate specification of retinal neurons and as an unlimited source of neural progenitors for treating degenerative diseases of the retina by cell replacement. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:177 / 184
页数:8
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