A highly Sensitive Turn-on Fluorescent Sensor for Ba2+ Based on G-Quadruplexes

被引：21

作者：

Xu, Lijun ^{[1
]}

Chen, Yang ^{[1
,2
]}

Zhang, Ruihua ^{[1
,2
]}

Gao, Tian ^{[1
]}

Zhang, Yajie ^{[1
]}

Shen, Xiaoqiang ^{[1
]}

Pei, Renjun ^{[1
]}

机构：

[1] Univ Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Chinese Acad Sci, Key Lab Nanobio Interface, Suzhou 215123, Peoples R China

[2] Xi An Jiao Tong Univ, Sch Pharm, Xian 710061, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2017年 / 27卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Barium; Fluorescence; G-quadruplexes; Label-free; Sensors; MASS-SPECTROMETRY; DNA; IONS; ACID; CATIONS; LEAD; RECOGNITION; SELECTIVITY; STABILITY; APTAMER;

D O I：

10.1007/s10895-016-1984-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A G-quadruplex-based fluorescent biosensor for highly sensitive detection of barium ion (Ba2+) was constructed for the first time. In the absence of Ba2+, the G-quadruplex-specific fluorescence ligand N-methyl mesoporphyrin IX (NMM) remained weakly fluorescent when coexisted with a single-stranded G-quadruplex sequence AGRO100. Upon addition of Ba2+, AGRO100 was folded into G-quadruplex structures with the aid of Ba2+, which bound with NMM by stacking forces and significantly enhanced its fluorescence. The maximum fluorescence intensity of NMM was increased by ca. 22-fold in response to 1 mu M Ba2+. This simple method exhibites a good linear relationship in the range of 0-600 nM with the detection limit of 4 nM. The detection method is turn-on, fast, economic, high in signal-to-noise ratio and free of participation of toxic organic solvents, demonstrating its great potential for on-site and real-time Ba2+ detection.

引用

页码：569 / 574

页数：6

共 34 条

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