Organoids as an in vitro model of human development and disease

被引：1032

作者：

Fatehullah, Aliya ^{[1
]}

Tan, Si Hui ^{[1
]}

Barker, Nick ^{[1
,2
,3
]}

机构：

[1] A STAR Inst Med Biol, 8A Biomed Grove,06-06 Immunos, Singapore 138648, Singapore

[2] Univ Edinburgh, Ctr Regenerat Med, 47 Little France Crescent, Edinburgh EH16 4TJ, Midlothian, Scotland

[3] Natl Univ Singapore, Dept Biochem, Yong Loo Lin Sch Med, Singapore 117596, Singapore

来源：

NATURE CELL BIOLOGY | 2016年 / 18卷 / 03期

关键词：

PLURIPOTENT STEM-CELLS; FUNCTIONAL HUMAN LIVER; DIRECTED DIFFERENTIATION; INTESTINAL ORGANOIDS; CEREBRAL ORGANOIDS; PROGENITOR CELLS; SINGLE LGR5; GENERATION; EXPANSION; CULTURE;

D O I：

10.1038/ncb3312

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The in vitro organoid model is a major technological breakthrough that has already been established as an essential tool in many basic biology and clinical applications. This near-physiological 3D model facilitates an accurate study of a range of in vivo biological processes including tissue renewal, stem cell/niche functions and tissue responses to drugs, mutation or damage. In this Review, we discuss the current achievements, challenges and potential applications of this technique.

引用

页码：246 / 254

页数：9

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