Hepatoprotective effect of a polyherbal formulation (Aab-e-Murawaqain) against CCl4 induced liver toxicity in Wistar albino rat model by suppressing proinflammatory cytokines

This study assessed the hepatoprotective action of Aab-e-Murawaqain in Wistar albino rats by inducing liver injury with carbon tetrachloride (CCl4) (2 mL/kg; sc). Aqueous extracts of Solanum nigrum (Makoy) and Cicho-rium intybus (Kashni) were used to prepare the Aab-e-Murawaqain polyherbal formulation. Silymarin (25 mg/kg), a well-known liver protective medicine, was treated as the reference control group. Biochemical indicators, such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transami-nase (SGPT), alkaline phosphatase (ALP), and total bilirubin, were used to assess the curative potential. Other common biomarkers, such as thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT), were also estimated. Animals treated with CCl4 exhibited significantly enhanced in SGOT, SGPT, ALP, bilirubin, and TBARS level, reflecting hepatic damage. The formulation consid-erably minimised the increase serum enzyme levels, indicating the improvement in liver cellular injury. CCl4 induced significant decrease in GSH, SOD, and CAT levels. Pretreatment of the rats with the formulation led to an increase in hepatic GSH, SOD, CAT, protein, and albumin levels. Moreover, CCl4 administration led to an increase in TNF-a, IL-6, and IL-10 levels, which decreased with polyherbal formulation treatment. The results were validated through histological evolutions, which revealed minimum fatty variations and a noticeable regenerative effect in the livers after treatment with the formulation. The results confirmed that Aab-e-Mur-awaqain can provide a substantial activity in the management of CCl4-induced hepatotoxicity.(c) 2021 SAAB. Published by Elsevier B.V. All rights reserved.