Derivation of keratinocyte progenitor cells and skin formation from embryonic stem cells

被引:50
作者
Aberdam, D [1 ]
机构
[1] Fac Med Nice, INSERM, U634, F-06107 Nice 2, France
关键词
mouse; embryonic stem cell; epidermal lineage; BMP-4; skin; mesenchyme;
D O I
10.1387/ijdb.15272386
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Despite numerous elegant transgenic mice experiments, the absence of an appropriate in vitro model system has hampered the study of the early events responsible for epidermal and dermal commitments. Embryonic stem (ES) cells are derived from the pluripotent cells of the early mouse embryo. They can be expanded infinitely in vitro while maintaining their potential to spontaneously differentiate into any cell type of the three germ layers, including epidermal cells. We recently reported that ES cells have the potential to recapitulate the reciprocal instructive ectodermal-mesodermal commitments, which are characteristic of embryonic skin formation. Derivation of epidermal cells from murine ES cells has been successfully established by exposing the cells to precisely controlled instructive influences normally found in the body, including extracellular matrix and the morphogen BMP-4. These differentiated ES cells are able to form, in culture, a multilayered epidermis coupled with an underlying dermal compartment similar to native skin. This bioengineered skin provides a powerful tool for studying the molecular mechanisms controlling skin development and epidermal stem cell properties.
引用
收藏
页码:203 / 206
页数:4
相关论文
共 50 条
[21]   Long-term culture of keratinocyte-like cells derived from mouse embryonic stem cells [J].
Hai-Jun Huang ;
Qi-Shuang Gao ;
Bi-Fei Tao ;
Si-Wen Jiang .
In Vitro Cellular & Developmental Biology - Animal, 2008, 44 :193-203
[22]   Hypoxia Enhances the Generation of Retinal Progenitor Cells from Human Induced Pluripotent and Embryonic Stem Cells [J].
Bae, Daekyeong ;
Mondragon-Teran, Paul ;
Hernandez, Diana ;
Ruban, Ludmila ;
Mason, Chris ;
Bhattacharya, Shomi S. ;
Veraitch, Farlan S. .
STEM CELLS AND DEVELOPMENT, 2012, 21 (08) :1344-1355
[23]   Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos [J].
Kawaguchi, Jitsutaro ;
Nichols, Jennifer ;
Gierl, Mathias S. ;
Faial, Tiago ;
Smith, Austin .
DEVELOPMENT, 2010, 137 (05) :693-704
[24]   Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts [J].
Bogliotti, Yanina Soledad ;
Wu, Jun ;
Vilarino, Marcela ;
Okamura, Daiji ;
Soto, Delia Alba ;
Zhong, Cuiqing ;
Sakurai, Masahiro ;
Sampaio, Rafael Vilar ;
Suzuki, Keiichiro ;
Belmonte, Juan Carlos Izpisua ;
Ross, Pablo Juan .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (09) :2090-2095
[25]   Progress of Differentiating Human Embryonic Stem Cells Into Endothelial Progenitor Cells and Potential Applications [J].
Wu Qiong ;
Xi Jia-Fei ;
Li Ya-Li ;
Pei Xue-Tao .
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2010, 37 (02) :138-144
[26]   Role of BMP Signaling in Pancreatic Progenitor Differentiation from Human Embryonic Stem Cells [J].
Lina Sui ;
Mieke Geens ;
Karen Sermon ;
Luc Bouwens ;
Josué Kunjom Mfopou .
Stem Cell Reviews and Reports, 2013, 9 :569-577
[27]   Derivation of extra-embryonic and intra-embryonic macrophage lineages from human pluripotent stem cells [J].
Bredemeyer, Andrea L. ;
Amrute, Junedh M. ;
Koenig, Andrew L. ;
Idol, Rachel A. ;
He, Li ;
Luff, Stephanie A. ;
Dege, Carissa ;
Leid, Jamison M. ;
Schilling, Joel D. ;
Hinson, J. Travis ;
Dinauer, Mary C. ;
Sturgeon, Christopher M. ;
Lavine, Kory J. .
DEVELOPMENT, 2022, 149 (08)
[28]   Generation of Murine Sympathoadrenergic Progenitor-Like Cells from Embryonic Stem Cells and Postnatal Adrenal Glands [J].
Saxena, Shobhit ;
Wahl, Joachim ;
Huber-Lang, Markus S. ;
Stadel, Dominic ;
Braubach, Peter ;
Debatin, Klaus-Michael ;
Beltinger, Christian .
PLOS ONE, 2013, 8 (05)
[29]   Role of BMP Signaling in Pancreatic Progenitor Differentiation from Human Embryonic Stem Cells [J].
Sui, Lina ;
Geens, Mieke ;
Sermon, Karen ;
Bouwens, Luc ;
Mfopou, Josue Kunjom .
STEM CELL REVIEWS AND REPORTS, 2013, 9 (05) :569-577
[30]   Development of hematopoietic cells from embryonic stem cells [J].
Suzuki, A ;
Nakano, T .
INTERNATIONAL JOURNAL OF HEMATOLOGY, 2001, 73 (01) :1-5