NOV is upregulated and promotes migration and invasion in bladder cancer

被引：6
作者：
Chen, Jie [1 ]
Gao, Yi [1 ]
Xu, Biyou [1 ]
Cui, Xingang [1 ]
Xu, Danfeng [1 ]
机构：
[1] Second Mil Med Univ, Changzheng Hosp, Dept Urol, Shanghai 200003, Peoples R China
来源：
TUMOR BIOLOGY | 2014年 / 35卷 / 07期
关键词：
Bladder cancer; Epithelial-mesenchymal transition; NOV; mTOR; EPITHELIUM-DERIVED FACTOR; MESENCHYMAL TRANSITION; TUMOR; EXPRESSION; ACTIVATION; MTORC1; EMT;
D O I：
10.1007/s13277-014-1919-8
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
NOV is pro-tumourigenic via epithelial-mesenchymal transition (EMT) in several malignancies but is not studied in bladder cancer (BCa). Whether NOV is responsible for bladder carcinogenesis and the underlying mechanism is unclear. Using immunohistochemical staining, we quantified expressions of NOV, pS6, Vimentin and E-cadherin in 66 bladder cancer and 10 normal bladder urothelium samples. EMT was profiled by EMT index (EMTi) calculated as the ratio of Vimentin to E-cadherin. In vitro and in vivo studies were carried out to profile the role of NOV in the tumourigenesis of BCa. NOV was upregulated in bladder cancer compared to normal tissue, and its expression was correlated to pS6 and EMTi. Expression of NOV was higher in recurrent and multiple tumours and was increased with progression of tumour grade. NOV expression was also higher in BCa cell lines. Silence of NOV attenuated EMT, decreased invasion and migration of BCa cells. Silence of NOV also inhibited xenograft tumour growth and decreased tumour EMT. NOV is pro-tumourigenic in bladder cancer especially in nonmuscle-invasive entities (NMIBC). NOV may promote carcinogenesis via promotion of EMT and association with increased mTOR activity.
引用
收藏
页码：6749 / 6755
页数：7
相关论文
共 22 条
[1]   NOV promoted the growth and migration of pancreatic cancer cells [J].
Cui, Lei ;
Xie, Rong ;
Dang, Shenchun ;
Zhang, Qing ;
Mao, Shengfa ;
Chen, Jixiang ;
Qu, Jianguo ;
Zhang, Jianxin .
TUMOR BIOLOGY, 2014, 35 (04) :3195-3201
[2]   Combination of Rapamycin, CI-1040, and 17-AAG Inhibits Metastatic Capacity of Prostate Cancer via Slug Inhibition [J].
Ding, Guanxiong ;
Feng, Chenchen ;
Jiang, Haowen ;
Ding, Qiang ;
Zhang, Limin ;
Na, Rong ;
Xu, Hua ;
Liu, Jun .
PLOS ONE, 2013, 8 (10)
[3]  
Feng C, 2012, Pathol Biol (Paris), V60, pe36, DOI 10.1016/j.patbio.2011.11.009
[4]  
Feng CC, 2011, FOLIA BIOL-PRAGUE, V57, P242
[5]   Expression of pigment epithelium-derived factor and tumor necrosis factor-α is correlated in bladder tumor and is related to tumor angiogenesis [J].
Feng, Chen-chen ;
Wang, Pao-hsun ;
Ding, Qiang ;
Guan, Ming ;
Zhang, Yuan-fang ;
Jiang, Hao-wen ;
Wen, Hui ;
Wu, Zhong .
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS, 2013, 31 (02) :241-246
[6]   Pigment epithelium-derived factor expression is down-regulated in bladder tumors and correlates with vascular endothelial growth factor and matrix metalloproteinase-9 [J].
Feng, Chen-chen ;
Ding, Qiang ;
Zhang, Yuan-fang ;
Jiang, Hao-wen ;
Wen, Hui ;
Wang, Pao-hsun ;
Wu, Zhong .
INTERNATIONAL UROLOGY AND NEPHROLOGY, 2011, 43 (02) :383-390
[7]   Expression of Pigment Epithelium-derived Factor in Bladder Tumour Is Correlated with Interleukin-8 yet Not with Interleukin-1α [J].
Feng, Chenchen ;
Guan, Ming ;
Ding, Qiang ;
Zhang, Yuanfang ;
Jiang, Haowen ;
Wen, Hui ;
Wang, Paohsun ;
Wu, Zhong .
JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2011, 31 (01) :21-25
[8]  
Ferley J, 2001, LARC CANC BASE
[9]   mTORC1 and mTORC2 Regulate EMT, Motility, and Metastasis of Colorectal Cancer via RhoA and Rac1 Signaling Pathways [J].
Gulhati, Pat ;
Bowen, Kanika A. ;
Liu, Jianyu ;
Stevens, Payton D. ;
Rychahou, Piotr G. ;
Chen, Min ;
Lee, Eun Y. ;
Weiss, Heidi L. ;
O'Connor, Kathleen L. ;
Gao, Tianyan ;
Evers, B. Mark .
CANCER RESEARCH, 2011, 71 (09) :3246-3256
[10]   The basics of epithelial-mesenchymal transition [J].
Kalluri, Raghu ;
Weinberg, Robert A. .
JOURNAL OF CLINICAL INVESTIGATION, 2009, 119 (06) :1420-1428
123