Analyzing Increased Fluorescence of Single Quantum Dots on Dehydrated Agarose by Single-Molecular Imaging

被引:0
|
作者
Wang, Jianfeng [1 ]
Liu, Wei [2 ]
Liu, Baoquan [1 ]
Fan, Shengdi [1 ]
Zhang, Ye-Wang [2 ]
机构
[1] Dalian Nationalities Univ, Sch Life Sci, Key Lab Biotechnol & Bioresources Utilizat, State Ethn Affairs Commiss,Minist Educ, Dalian, Peoples R China
[2] Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China
关键词
dehydrated agarose; quantum dots; single-molecular imaging; CDSE/CDS CORE/SHELL NANOCRYSTALS; SEMICONDUCTOR NANOCRYSTALS; BLINKING; INTERMITTENCY; PHOTOLUMINESCENCE; LUMINESCENCE; SUPPRESSION; DIFFUSION; KINETICS;
D O I
10.1080/00387010.2012.700544
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
A single-molecule imaging technique was used to study emission behaviors of quantum dots (QDs) on the surface of glass and dehydrated agarose. The luminescence intensity of single QDs on the dehydrated agarose film was 2 or 3 times that on the glass. Our results revealed that the roughness and the hydrophobic nature of dehydrated agarose gel can suppress the blinking of single QDs. A clear decrease in the power law exponent for on time but an increase for off time was observed when the glass was replaced with 4% and 8% dehydrated agarose. The enhanced luminescence of QDs on dehydrated agarose will make their applications on microarrays more attractive.
引用
收藏
页码:195 / 200
页数:6
相关论文
共 50 条
  • [31] Single-molecular diodes based on opioid derivatives
    M. R. S. Siqueira
    S. M. Corrêa
    R. M. Gester
    J. Del Nero
    A. M. J. C. Neto
    Journal of Molecular Modeling, 2015, 21
  • [32] Effectively blocked mechanism in quantum tunnelling of n-coupled single-molecular magnets
    Chen, ZD
    CHINESE PHYSICS LETTERS, 2005, 22 (10) : 2663 - 2665
  • [33] Single molecule localization imaging of telomeres and centromeres using fluorescence in situ hybridization and semiconductor quantum dots
    Wang, Le
    Zong, Shenfei
    Wang, Zhuyuan
    Lu, Ju
    Chen, Chen
    Zhang, Ruohu
    Cui, Yiping
    NANOTECHNOLOGY, 2018, 29 (28)
  • [34] Probing dynamic fluorescence properties of single and clustered quantum dots toward quantitative biomedical imaging of cells
    Kang, Hyeong-Gon
    Tokumasu, Fuyuki
    Clarke, Matthew
    Zhou, Zhenping
    Tang, Jianyong
    Nguyen, Tinh
    Hwang, Jeeseong
    WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY, 2010, 2 (01) : 48 - 58
  • [35] Multifunctional silanized quantum dots: From single molecule fluorescence to magnetic resonance imaging probes.
    Gerion, D
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 229 : U641 - U641
  • [36] Bio-MEMS towards Single-Molecular Characterization
    Fujita, Hiroyuki
    2014 IEEE INTERNATIONAL ELECTRON DEVICES MEETING (IEDM), 2014,
  • [37] Single-molecular surface-induced fluorescence attenuation based on thermal reduced graphene oxide
    Fan, Qin-Kai
    Yang, Chen-Guang
    Hu, Shu-Xin
    Xu, Chun-Hua
    Li, Ming
    Lu, Ying
    ACTA PHYSICA SINICA, 2023, 72 (14)
  • [38] Functional oligothiophenes toward molecular wires in single-molecular electronics
    Ie, Yutaka
    Endou, Masaru
    Han, Aihong
    Yamada, Ryo
    Tada, Hirokazu
    Aso, Yoshio
    PURE AND APPLIED CHEMISTRY, 2012, 84 (04) : 931 - 943
  • [39] Single Molecule Imaging with Stable 6 NM Quantum Dots
    Cai, En
    Wilson, Katie
    Ge, Pinghua
    Tjioe, Marco
    Fernig, David G.
    Selvin, Paul R.
    BIOPHYSICAL JOURNAL, 2012, 102 (03) : 182A - 182A
  • [40] Quantum dots for quantitative imaging: from single molecules to tissue
    Vu, Tania Q.
    Lam, Wai Yan
    Hatch, Ellen W.
    Lidke, Diane S.
    CELL AND TISSUE RESEARCH, 2015, 360 (01) : 71 - 86