Optimization for ultrasound-assisted extraction of polysaccharides with antioxidant activity in vitro from the aerial root of Ficus microcarpa

被引:59
作者
Jiang, Changxing [1 ,4 ]
Li, Xia [2 ]
Jiao, Yunpeng [3 ]
Jiang, Dingyun [1 ]
Zhang, Ling [1 ]
Fan, Benxia [1 ]
Zhang, Qianghua [1 ]
机构
[1] Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian 223003, Jiangsu, Peoples R China
[2] Xuzhou Med Coll, Affiliated Huaian Hosp, Huaian 223002, Jiangsu, Peoples R China
[3] Jiangsu Food & Pharmaceut Sci Coll, Dept Food Engn, Huaian 223003, Jiangsu, Peoples R China
[4] Huaiyin Inst Technol, Jiangsu Prov Engn Lab Biomass Convers & Proc Inte, Huaian 223003, Jiangsu, Peoples R China
关键词
Polysaccharide; Ficus micrompa; Ultrasound-assisted extraction; Response surface methodology; Antioxidant activity; RESPONSE-SURFACE METHODOLOGY; PURIFICATION; L;
D O I
10.1016/j.carbpol.2014.03.027
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this study, optimization of ultrasound-assisted extraction and antioxidant activity of polysaccharides from the aerial root of Ficus microcarpa (FMPS) were investigated. The optimal conditions for extraction of FMPS were determined as followings: ultrasound power 200W, ultrasound temperature 70 degrees C, extraction temperature 74 degrees C, liquid-solid ratio 35, extraction time 238 min, ultrasound time 49 min. The experimental yield of FMPS (3.44%) obtained under these conditions was well agreement with the value predicted by the model. In addition, Fourier transform-infrared spectroscopy and antioxidant activity assays revealed that FMPS were acidic polysaccharides and had strong Fe2+ chelating activity and moderate hydrogen peroxide scavenging effect. Further work on the purification, structure characterization and antioxidant activity in vivo of FMPS is in progress. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:10 / 17
页数:8
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