Flow-injection determination of vitamin A in pharmaceutical formulations using Tris(2,2′-bipyridyl)Ru(II)-Ce(IV) chemiluminescence detection

被引：18

作者：

Yaqoob, Mohammad ^{[1
]}

Waseem, Amir ^{[1
]}

Rishi, Lubna ^{[1
]}

Nabi, Abdul ^{[1
]}

机构：

[1] Univ Balochistan, Dept Chem, Quetta, Pakistan

来源：

LUMINESCENCE | 2009年 / 24卷 / 05期

关键词：

flow injection; chemiluminescence; tris(2,2 '-bipyridyl)Ru(III); vitamin A; pharmaceutical formulations; LIQUID-CHROMATOGRAPHY; ALPHA-TOCOPHEROL; PUBLIC-HEALTH; TRIS(2,2'-BIPYRIDYL)RUTHENIUM(III); CAROTENOIDS; RETINOL; VALIDATION; THYROXINE; ELECTRONS; ACCEPTORS;

D O I：

10.1002/bio.1089

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow injection method with chemiluminescence detection is reported for the determination of vitamin A. The method is based on the enhancement effect of vitamin A on chemiluminescence of tris(2,2'-bipyridyl)Ru(II)-Ce(IV) in acidic medium. The proposed procedure is used to quantitate vitamin A in the range 1.0-100 x 10(-6) mol/L with a correlation coefficient of 0.9991 (n = 9) and relative standard deviation in the range 1.2-2.3% (n = 4). The limit of detection (3 x blank) was 8.0 x 10(-8) mol/L with a sample throughput of 100/h. The effect of common excipients used in pharmaceutical formulations and some clinically important compounds was also studied. The method was applied to determine vitamin A in pharmaceutical formulations and the results obtained were in reasonable agreement with the amount quoted. The results were compared using spectrophotometric method and no significant difference was found between the results of the two methods at 95% confidence limit. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：276 / 281

页数：6

共 39 条

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