A Panel of Oxidative Stress Markers in Parkinson's Disease

被引:33
作者
Bolner, A. [1 ]
Micciolo, R. [2 ]
Bosello, O. [3 ]
Nordera, Gp. [1 ]
机构
[1] Casa Cura Villa Margherita, Oxidat Stress Ctr CSOx, Vicenza, Italy
[2] Univ Trento, Dept Psychol & Cognit Sci, Trento, Italy
[3] Univ Verona, Dept Med, I-37100 Verona, Italy
关键词
oxidative stress; markers; Parkinson's disease; LIPID-PEROXIDATION; SUBSTANTIA-NIGRA; CELL-DEATH; HPLC; NITRATION; PLASMA; IRON;
D O I
10.7754/Clin.Lab.2015.150538
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Oxidative stress may be the cause or effect of several pathogenetic processes such as neurodegenerative diseases. The aim of this paper was to evaluate the diagnostic efficacy in Parkinson's disease (PKD) of a panel of oxidative stress markers selected from the many proposed by the most recent literature. Methods: 23 molecules including both plasma and urinary oxidative markers such total radical oxygen species, homocysteine, biological antioxidant potential, glutathione, superoxide dismutase, uric acid, total bilirubin, iron, ferritin, coenzyme Q10, 3-nitrotyrosine, total lipoperoxides, 4-hydroxy-nonenal, and 8-hydroxy-deoxy-guanosine were determined both in PKD and aged control subjects. For each analyte and group, the respective reference intervals were determined. Statistical analysis was used to assess the existence of significant differences between intervals in order to indicate which markers can better characterize PKD and distinguish it from the control population. Results: Some parameters were different in both groups when compared to those observed in younger subjects, supporting the hypothesis that aging is associated with an increase of oxidative stress. A peculiar increase of oxidative damage on nucleic acids was found in PKD, as well as a less efficient turnover of the DNA and an increase of protein peroxidation. Conclusions: Our results demonstrate that in PKD there is an increase of oxidative attack on nucleic acids and that the protein nitration is a characteristic phenomenon. These observations are in good agreement with the hypothesis that in PKD oxidative damage occurs that counter-regulatory systems attempt to balance, but inefficiently.
引用
收藏
页码:105 / 112
页数:8
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