Nicorandil improves post-fatigue tension in slow skeletal muscle fibers by modulating glutathione redox state

被引:14
作者
Sanchez-Duarte, E. [1 ]
Trujillo, X. [1 ]
Cortes-Rojo, C. [2 ]
Saavedra-Molina, A. [2 ]
Camargo, G. [3 ]
Hernandez, L. [3 ]
Huerta, M. [1 ]
Montoya-Perez, R. [2 ]
机构
[1] Univ Colima, Ctr Univ Invest Biomed, Ave 25 Julio 985 Col Villa San Sebastian, Colima 28040, Colima, Mexico
[2] Univ Michoacana, Inst Invest Quim Biol, Francisco J Mujica S-N, Morelia 58030, Michoacan, Mexico
[3] Univ Guadalajara, Ctr Univ Ciencias Dela Salud, Dept Fisiol, Lab Neurofisiol, Sierra Mojada 950, Guadalajara 44340, Jalisco, Mexico
关键词
Fatigue; Skeletal muscle; Nicorandil; Reactive oxygen species; Nitric oxide; K-ATP CHANNELS; SENSITIVE POTASSIUM CHANNELS; ISCHEMIA-REPERFUSION INJURY; NITRIC-OXIDE SYNTHASE; REACTIVE OXYGEN; MITOCHONDRIAL-FUNCTION; CONTRACTILE FUNCTION; OXIDATIVE STRESS; EXERCISE; ROS;
D O I
10.1007/s10863-016-9692-6
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Fatigue is a phenomenon in which force reduction has been linked to impairment of several biochemical processes. In skeletal muscle, the ATP-sensitive potassium channels (K-ATP) are actively involved in myoprotection against metabolic stress. They are present in sarcolemma and mitochondria (mitoK(ATP) channels). K+ channel openers like nicorandil has been recognized for their ability to protect skeletal muscle from ischemia-reperfusion injury, however, the effects of nicorandil on fatigue in slow skeletal muscle fibers has not been explored, being the aim of this study. Nicorandil (10 mu M), improved the muscle function reversing fatigue as increased post-fatigue tension in the peak and total tension significantly with respect to the fatigued condition. However, this beneficial effect was prevented by the mitoK(ATP) channel blocker 5-hydroxydecanoate (5-HD, 500 mu M) and by the free radical scavenger N-2-mercaptopropionyl glycine (MPG, 1 mM), but not by the nitric oxide (NO) synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME, 100 mu M). Nicorandil also decreased lipid peroxidation and maintained both reduced glutathione (GSH) levels and an elevated GSH/GSSG ratio, whereas total glutathione (TGSH) remained unaltered during post-fatigue tension. In addition, NO production, measured through nitrite concentrations was significantly increased with nicorandil during post-fatigue tension; this increase remained unaltered in the presence of nicorandil plus L-NAME, nonetheless, this effect was reversed with nicorandil plus MPG. Hence, these results suggest that nicorandil improves the muscle function reversing fatigue in slow skeletal muscle fibers of chicken through its effects not only as a mitoK(ATP) channel opener but also as NO donor and as an antioxidant.
引用
收藏
页码:159 / 170
页数:12
相关论文
共 74 条
[51]   Reactive oxygen and nitrogen species as intracellular signals in skeletal muscle [J].
Powers, Scott K. ;
Talbert, Erin E. ;
Adhihetty, Peter J. .
JOURNAL OF PHYSIOLOGY-LONDON, 2011, 589 (09) :2129-2138
[52]   Levels of biological markers of nitric oxide in serum of patients with squamous cell carcinoma of the oral cavity [J].
Ratajczak-Wrona, Wioletta ;
Jablonska, Ewa ;
Antonowicz, Bozena ;
Dziemianczyk, Dorota ;
Grabowska, Stanislawa Zyta .
INTERNATIONAL JOURNAL OF ORAL SCIENCE, 2013, 5 (03) :141-145
[53]   Reactive Oxygen Species as Agents of Fatigue [J].
Reid, Michael B. .
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 2016, 48 (11) :2239-2246
[54]   Redefining the major contributors to superoxide production in contracting skeletal muscle. The role of NAD(P)H oxidases [J].
Sakellariou, G. K. ;
Jackson, M. J. ;
Vasilaki, A. .
FREE RADICAL RESEARCH, 2014, 48 (01) :12-29
[55]   Cardioprotective Effect of Nicorandil, a Mitochondrial ATP-Sensitive Potassium Channel Opener, Prolongs Survival in HSPB5 R120G Transgenic Mice [J].
Sanbe, Atsushi ;
Marunouchi, Tetsuro ;
Yamauchi, Junji ;
Tanonaka, Kouichi ;
Nishigori, Hideo ;
Tanoue, Akito .
PLOS ONE, 2011, 6 (04)
[56]  
Sanchez-Duarte E., 2012, Open Access Animal Physiology, V4, P21
[57]   Nicorandil, a potent cardioprotective agent, acts by opening mitochondrial ATP-dependent potassium channels [J].
Sato, T ;
Sasaki, N ;
O'Rourke, B ;
Marbán, E .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2000, 35 (02) :514-518
[58]   Effects of nitric oxide donor and inhibitor on prostaglandin E2-like activity, malondialdehyde and reduced glutathione levels after skeletal muscle ischemia-reperfusion [J].
Sayan, H ;
Babül, A ;
Ugurlu, B .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 2001, 65 (04) :179-183
[59]   Changes in myoplasmic Ca2+ during fatigue differ between FDB fibers, between glibenclamide-exposed and Kir6.2(-/-) fibers and are further modulated by verapamil [J].
Selvin, David ;
Renaud, Jean-Marc .
PHYSIOLOGICAL REPORTS, 2015, 3 (03)
[60]   Nicorandil prevents endothelial dysfunction due to antioxidative effects via normalisation of NADPH oxidase and nitric oxide synthase in streptozotocin diabetic rats [J].
Serizawa, Ken-ichi ;
Yogo, Kenji ;
Aizawa, Ken ;
Tashiro, Yoshihito ;
Ishizuka, Nobuhiko .
CARDIOVASCULAR DIABETOLOGY, 2011, 10