CRISPR/Cas9 Initiated Transgenic Silkworms as a Natural Spinner of Spider Silk

被引：63

作者：

Zhang, Xiaoli ^{[1
]}

Xia, Lijin ^{[1
]}

Day, Breton A. ^{[1
,2
]}

Harris, Thomas I. ^{[3
]}

Oliveira, Paula ^{[1
]}

Knittel, Chelsea ^{[4
]}

Licon, Ana Laura ^{[3
]}

Gong, Chengliang ^{[5
]}

Dion, Genevieve ^{[6
]}

Lewis, Randolph V. ^{[1
,3
]}

Jones, Justin A. ^{[1
]}

机构：

[1] Utah State Univ, Dept Biol, Logan, UT 84341 USA

[2] Utah State Univ, Dept Nutr Dietet & Food Sci, Logan, UT 84341 USA

[3] Utah State Univ, Dept Biol Engn, Logan, UT 84341 USA

[4] Drexel Univ, Coll Engn, Philadelphia, PA 19104 USA

[5] Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Peoples R China

[6] Drexel Univ, Westphal Coll Media Arts & Design, Philadelphia, PA 19104 USA

来源：

BIOMACROMOLECULES | 2019年 / 20卷 / 06期

关键词：

STRAND BREAK REPAIR; BOMBYX-MORI; DRAGLINE SILK; HOMOLOGOUS RECOMBINATION; FIBERS SPUN; GENE; PROTEINS; FIBROIN; GENOME; EXPRESSION;

D O I：

10.1021/acs.biomac.9b00193

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using transgenic silkworms with their natural spinning apparatus has proven to be a promising way to spin spider silk-like fibers. The challenges are incorporating native-size spider silk proteins and achieving an inheritable transgenic silkworm strain. In this study, a CRISPR/Cas9 initiated fixed-point strategy was used to successfully incorporate spider silk protein genes into the Bombyx mori genome. Native-size spider silk genes (up to 10 kb) were inserted into an intron of the fibroin heavy or light chain (FibH or FibL) ensuring that any sequence changes induced by the CRISPR/Cas9 would not impact protein production. The resulting fibers are as strong as native spider silks (1.2 GPa tensile strength). The transgenic silkworms have been tracked for several generations with normal inheritance of the transgenes. This strategy demonstrates the feasibility of using silkworms as a natural spider silk spinner for industrial production of high-performance fibers.

引用

页码：2252 / 2264

页数：13

共 53 条

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