Molecular, phenotypic, and sample-associated data to describe pluripotent stem cell lines and derivatives

被引：50

作者：

Daily, Kenneth ^{[1
]}

Sui, Shannan J. Ho ^{[2
]}

Schriml, Lynn M. ^{[3
,4
]}

Dexheimer, Phillip J. ^{[5
]}

Salomonis, Nathan ^{[5
]}

Schroll, Robin ^{[6
]}

Bush, Stacy ^{[6
]}

Keddache, Mehdi ^{[7
]}

Mayhew, Christopher ^{[8
]}

Lotia, Samad ^{[9
]}

Perumal, Thanneer M. ^{[1
]}

Dang, Kristen ^{[1
]}

Pantano, Lorena ^{[2
]}

Pico, Alexander R. ^{[9
]}

Grassman, Elke ^{[10
]}

Nordling, Diana ^{[11
]}

Hide, Winston ^{[2
,12
,13
]}

Hatzopoulos, Antonis K. ^{[14
,15
]}

Malik, Punam ^{[3
,4
]}

Cancelas, Jose A. ^{[6
]}

Lutzko, Carolyn ^{[6
]}

Aronow, Bruce J. ^{[5
]}

Omberg, Larsson ^{[1
]}

机构：

[1] Sage Bionetworks, Seattle, WA 98109 USA

[2] Harvard TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA

[3] Univ Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21201 USA

[4] Univ Maryland, Sch Med, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA

[5] Cincinnati Childrens Hosp, Dept Biomed Informat, Cincinnati, OH 45229 USA

[6] Cincinnati Childrens Hosp, Div Expt Hematol & Canc Biol, Cincinnati, OH 45229 USA

[7] Cincinnati Childrens Hosp, Div Human Genet, Cincinnati, OH 45229 USA

[8] Cincinnati Childrens Hosp, Div Dev Biol, Cincinnati, OH 45229 USA

[9] Gladstone Inst, San Francisco, CA 94158 USA

[10] Cincinnati Childrens Hosp, Translat Trials Dev & Support Lab Field Serv, Cincinnati, OH 45229 USA

[11] Cincinnati Childrens Hosp, Cell Manipulat Lab, Cincinnati, OH 45229 USA

[12] Univ Sheffield, Sheffield Inst Translat Neurosci, Sheffield S10 2HQ, S Yorkshire, England

[13] Harvard Stem Cell Inst, Cambridge, MA 02138 USA

[14] Vanderbilt Univ, Dept Med, Div Cardiovasc Med, Nashville, TN 37232 USA

[15] Vanderbilt Univ, Dept Cell & Dev Biol, Div Cardiovasc Med, Nashville, TN 37232 USA

来源：

SCIENTIFIC DATA | 2017年 / 4卷

关键词：

GENE; DIFFERENTIATION; FORMAT; SUITE; KEGG;

D O I：

10.1038/sdata.2017.30

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The use of induced pluripotent stem cells (iPSC) derived from independent patients and sources holds considerable promise to improve the understanding of development and disease. However, optimized use of iPSC depends on our ability to develop methods to efficiently qualify cell lines and protocols, monitor genetic stability, and evaluate self-renewal and differentiation potential. To accomplish these goals, 57 stem cell lines from 10 laboratories were differentiated to 7 different states, resulting in 248 analyzed samples. Cell lines were differentiated and characterized at a central laboratory using standardized cell culture methodologies, protocols, and metadata descriptors. Stem cell and derived differentiated lines were characterized using RNA-seq, miRNA-seq, copy number arrays, DNA methylation arrays, flow cytometry, and molecular histology. All materials, including raw data, metadata, analysis and processing code, and methodological and provenance documentation are publicly available for re-use and interactive exploration at https://www.synapse.org/pcbc. The goal is to provide data that can improve our ability to robustly and reproducibly use human pluripotent stem cells to understand development and disease.

引用

页数：10

共 32 条

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