Essential role of the tyrosine kinase substrate phospholipase C-gamma 1 in mammalian growth and development

被引：212
作者：
Ji, QS
Winnier, GE
Niswender, KD
Horstman, D
Wisdom, R
Magnuson, MA
Carpenter, G
机构：
[1] VANDERBILT UNIV,SCH MED,DEPT BIOCHEM,NASHVILLE,TN 37232
[2] VANDERBILT UNIV,SCH MED,DEPT CELL BIOL,NASHVILLE,TN 37232
[3] VANDERBILT UNIV,SCH MED,DEPT PHYSIOL & MOL BIOPHYS,NASHVILLE,TN 37232
[4] VANDERBILT UNIV,SCH MED,DEPT MED,NASHVILLE,TN 37232
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 07期
关键词：
D O I：
10.1073/pnas.94.7.2999
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The activation of many tyrosine kinases leads to the phosphorylation and activation of phospholipase C-gamma 1 (PLC-gamma 1), To examine the biological function of this protein, homologous recombination has been used to selectively disrupt the Plcg1 gene in mice, Homozygous disruption of Plcg1 results in embryonic lethality at approximately embryonic day (E) 9.0, Histological analysis Indicates that Plcg1 (-/-) embryos appear normal at E 8.5 but fail to continue normal development and growth beyond E 8.5-E9.0. These results clearly demonstrate that PLC-gamma 1 with, by inference, its capacity to mobilize second messenger molecules is an essential signal transducing molecule whose absence is not compensated by other signaling pathways or other genes encoding PLC isozymes.
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页码：2999 / 3003
页数：5
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