An extracellular Bacillus sp chitinase for the production of chitotriose as a major chitinolytic product

被引：31

作者：

Woo, CJ ^{[1
]}

Park, HD ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Dept Food Sci & Technol, Taegu 702701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 05期

关键词：

Bacillus sp; chitinase; chitooligosaccharide; chitotriose; purification;

D O I：

10.1023/A:1022493226727

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An extracellular chitinase of Bacillus sp. WY22 was purified by 9.6-fold. It had a Mr of 35 kDa, an apparent K-m value for colloidal chitin of 3 mg ml(-1) and was optimally active at 37 degreesC and pH 5.5 over 1 h. The enzyme could also hydrolyse swollen chitin, glycol chitin and chitosan with relative activities of 76%, 34% and 23% compared with colloidal chitin. It formed chitotriose as a major product from colloidal chitin and glycol chitin.

引用

页码：409 / 412

页数：4

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