Subunit specificity and organ distribution of glutathione transferase-catalysed S-nitrosoglutathione formation from alkyl nitrites in the rat

被引：11
作者：
Akerboom, TPM
Ji, YB
Wagner, G
Sies, H
机构：
[1] Inst. fur Physiologische Chem. I, Heinrich-Heine-Univ. Düsseldorf, Postfach 101007
来源：
BIOCHEMICAL PHARMACOLOGY | 1997年 / 53卷 / 01期
关键词：
S-nitrosoglutathione; glutathione transferase; amyl nitrite; n-butyl nitrite;
D O I：
10.1016/S0006-2952(96)00602-8
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Glutathione transferase (GST)-catalysed S-nitrosoglutathione (GSNO) formation from alkyl nitrites was determined with the homodimers 1-1, 2-2, 3-3, and 4-4 isolated from rat liver. The 4-4 isoform showed a high specificity for the alkyl nitrites. Total GST activities were studied in homogenates from different organs. The liver showed highest GST activity both with amyl nitrite and with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, the activity ratio of amyl nitrite over CDNB being 3.8. In lung and heart, these ratios were 6.2 and 5.7, respectively, indicating a selectivity of these organs for alkyl nitrite metabolism and GSNO formation. Copyright (C) 1996 Elsevier Science Inc.
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收藏
页码：117 / 120
页数：4
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