Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato

被引：17

作者：

Shandil, Rajesh K. ^{[1
]}

Chakrabarti, Swarup K. ^{[1
]}

Singh, Bir Pal ^{[1
]}

Sharma, Sanjeev ^{[1
]}

Sundaresha, S. ^{[1
]}

Kaushik, Surinder K. ^{[2
]}

Bhatt, Arvind K. ^{[3
]}

Sharma, Nitya Nand ^{[4
]}

机构：

[1] ICAR Cent Potato Res Inst, Shimla 171001, Himachal Prades, India

[2] ICAR Natl Bur Plant Genet Resources, Div Germplasm Evaluat, Pusa Campus, New Delhi, India

[3] Himachal Pradesh Univ, Dept Biotechnol, Shimla, HP, India

[4] Premas BiotechPvt Ltd, Gurgaon, Haryana, India

来源：

BMC GENETICS | 2017年 / 18卷

关键词：

RB gene; Phytophthora infestans; Molecular breeding; Transgenic potato; BROAD-SPECTRUM RESISTANCE; PHYTOPHTHORA-INFESTANS; SOLANUM-BULBOCASTANUM; TRANSGENIC POTATO; EXPRESSION; TRANSCRIPT; LINES;

D O I：

10.1186/s12863-017-0490-x

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Late blight, caused by oomycetes pathogen Phytophthora infestans (Mont.) de Bary, is the most devastating potato disease in the world. RB gene from Solanum bulbocastanum has been shown to impart broad spectrum resistance against P. infestans races. In this study Katahdin transgenic event SP951 was used as male parent to cross with the popular Indian potato cultivars viz., Kufri Bahar (KB) and Kufri Jyoti (KJ) to enhance the late blight resistance. Results: Populations of 271 F1seedlings from the crosses KB x SP951 (87) and KJ x SP951 (184) were screened for inheritance of RB transgene through PCR and bioassay. Disease response based on AUDPC of different hybrid lines varied from immunity to complete susceptibility. High degree of resistance (< 25% infection) was observed in KJ x SP951 derived seedlings (85.2%), whereas level of resistance in KB x SP951 (36.4% infection) derived seedlings was of low order. Conclusion: This study provides valuable genetic materials for development of potentially durable late blight resistant potato varieties. Besides, it also corroborates the fact that efficacy of R gene is not solely dependent on its presence in the variety but largely depends on the genetic background of the recipient genotype.

引用

页数：8

共 23 条

[11] Correlation Between Transcript Abundance of the RB Gene and the Level of the RB-Mediated Late Blight Resistance in Potato [J].

Kramer, Lara C. ;

Choudoir, Mallory J. ;

Wielgus, Susan M. ;

Bhaskar, Pudota B. ;

Jiang, Jiming .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2009, 22 (04) :447-455

[12] Late blight resistance of RB Transgenic potato lines [J].

Kuhl, Joseph C. ;

Zarka, Kelly ;

Coombs, Joseph ;

Kirk, William W. ;

Douches, David S. .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2007, 132 (06) :783-789

[13] Tobacco mosaic virus regulates the expression of its own resistance gene N [J].

Levy, M ;

Edelbaum, O ;

Sela, I .

PLANT PHYSIOLOGY, 2004, 135 (04) :2392-2397

[14] ASSESSMENT OF FIELD-RESISTANCE TO BLIGHT (PHYTOPHTHORA-INFESTANS) IN POTATOES [J].

MALCOLMSON, JF .

TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY, 1976, 67 (OCT) :321-325

[15] Development of allele-specific PCR and RT-PCR assays for clustered resistance genes using a potato late blight resistance transgene as a model [J].

Millett, B. P. ;

Bradeen, J. M. .

THEORETICAL AND APPLIED GENETICS, 2007, 114 (03) :501-513

[16] Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines [J].

Naess S. ;

Bradeen J. ;

Wielgus S. ;

Haberlach G. ;

McGrath J. ;

Helgeson J. .

Molecular Genetics and Genomics, 2001, 265 (4) :694-704

[17]

Sambrook J., 1989, Molecular Cloning: A Laboratory, V2

[18] EFFECT OF NITROGEN-FERTILIZATION ON EXPRESSION OF SLOW-MILDEWING RESISTANCE IN KNOX WHEAT [J].

SHANER, G ;

FINNEY, RE .

PHYTOPATHOLOGY, 1977, 67 (08) :1051-1056

[19]

Singh B. P., 1994, POTATO PRESENT FUTUR, P179

[20] Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight [J].

Song, JQ ;

Bradeen, JM ;

Naess, SK ;

Raasch, JA ;

Wielgus, SM ;

Haberlach, GT ;

Liu, J ;

Kuang, HH ;

Austin-Phillips, S ;

Buell, CR ;

Helgeson, JP ;

Jiang, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (16) :9128-9133

← 1 2 3 →