Development of an immunochromatographic assay for rapid detection of 1-Aminohydantoin in urine specimens

被引：21

作者：

Xu, Yi Ping ^{[1
]}

Liu, Li Qiang ^{[1
]}

Li, Qiu Shen ^{[1
]}

Peng, Chi Fang ^{[1
]}

Chen, Wei ^{[1
]}

Xu, Chuan Lai ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, Jiangsu 214000, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2009年 / 23卷 / 03期

关键词：

1-aminohydantoin; immunochromatographic assay; colloidal gold; test strip; urine; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; NITROFURAN ANTIBIOTICS; ENZYME-IMMUNOASSAY; FURAZOLIDONE; TISSUE; METABOLITES; DEPLETION; RESIDUES; STRIP;

D O I：

10.1002/bmc.1115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid immunochromatographic assay was developed and validated for detection of 1-aminohydantoin (AHD) in urine specimens. Colloidal gold-labeled polyclonal antibody specific to AHD derivative was used as the marker, based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative-conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL(-1) and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme-linked immunosorbent assay. The semi-quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofurain residues. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：308 / 314

页数：7

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