Adipose stem cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity via the IGF-1/PI3K/AKT pathway

被引：9
作者：
Wang, Xianjun [1 ]
Zhao, Zhenyu [1 ]
Gong, Jian [1 ]
Zhou, Shengnian [2 ,3 ]
Peng, Hongjun [4 ]
Shatara, Adam [5 ]
Zhu, Timmy Z. [5 ]
Meltzer, Rebecca [5 ]
Du, Yansheng [5 ]
Gu, Huiying [5 ]
机构：
[1] Linyi Peoples Hosp, Dept Neurol, Linyi 276000, Shandong, Peoples R China
[2] Shandong Univ, Qilu Hosp, Dept Neurol, Jinan 250012, Shandong, Peoples R China
[3] Shandong Univ, Bain Sci Res Inst, Jinan 250012, Shandong, Peoples R China
[4] Nanjing Univ, Sch Med, Jinling Hosp, Dept Pediat, Nanjing 210002, Jiangsu, Peoples R China
[5] Indiana Univ Sch Med, Dept Neurol, Indianapolis, IN 46202 USA
来源：
NEUROSCIENCE LETTERS | 2014年 / 581卷
关键词：
Neuronal cell death; ASC-CM; 6-hydroxydopamine; IGF-1; AKT; CEREBELLAR GRANULE NEURONS; HEPATOCYTE GROWTH-FACTOR; STROMAL CELLS; PARKINSONS-DISEASE; RAT MODEL; IN-VITRO; CYSTEINE PROTEASE; IFATS COLLECTION; IGF-I; DIFFERENTIATION;
D O I：
10.1016/j.neulet.2014.08.033
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Previous studies suggest that the delivery of neurotrophic factors secreted from adipose stromal cells (ASC) protect the brain from 6-hydroxydopamine (6-OHDA)-induced neurotoxicity. However, it remains unclear which secreted neurotrophic factor has an important role in protecting 6-OHDA-treated neurons. Through the use of antibodies in this study, we demonstrated that specific neutralization of IGF-1 activity in ASC conditioned media (ASC-CM) significantly blocks ASC-CM-induced neuroprotection against 6-OHDA neurotoxicity. Consistently, this neuroprotection was mostly attributed to the activation of the AKT-mediated signaling pathway. In contrast, brain derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in ASC-CM did not play a role in ASC-CM-induced neuroprotection against 6-OHDA. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：98 / 102
页数：5
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