Glucose-Sensitive Microcapsules from Glutaraldehyde Cross-Linked Hemoglobin and Glucose Oxidase

被引：99
作者：
Qi, Wei [1 ]
Yan, Xuehai [1 ,4 ]
Juan, Li [1 ,3 ]
Cui, Yue [1 ,4 ]
Yang, Yang [2 ]
Li, Junbai [1 ]
机构：
[1] Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid & Interface Sci, Beijing Natl Lab Mol Sci,Int Joint Lab, Beijing 100190, Peoples R China
[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
[3] NW Inst Nucl Technol, Xian 710024, Peoples R China
[4] Max Planck Inst Colloids & Interfaces, D-14476 Golm, Germany
来源：
BIOMACROMOLECULES | 2009年 / 10卷 / 05期
关键词：
POLYELECTROLYTE MICROCAPSULES; CAPSULES; PEROXIDASE; RELEASE; FILMS; PH; MULTILAYERS; PARTICLES; STABILITY; CARBONATE;
D O I：
10.1021/bm801502r
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have fabricated the glucose-sensitive protein hollow microcapsules from hemoglobin (Hb) and glucose oxidase (GOx) via layer-by-layer technique with glutaraldehyde (GA) as a cross-linking agent. We demonstrated the coupled enzymatic reactions between Hb and GOx within capsules by means of CLSM. The real-time monitoring of the consumption of glucose may be useful for the design of a glucose sensor. Furthermore, to further study the effect of glucose on the capsules, we investigated the wall permeability of (Hb/GOx)(5) capsules. It was found that the glucose consumption could enhance the permeability of (Hb/GOx)(5) capsules. This is significant for the development of advanced drug delivery devices. Therefore, the fabricated Hb/GOx capsules may be a potential candidate for the creation of multifunctional devices.
引用
收藏
页码：1212 / 1216
页数：5
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