Microfluidic technologies and devices for lipid nanoparticle-based RNA delivery

被引：197

作者：

Maeki, Masatoshi ^{[1
,2
]}

Uno, Shuya ^{[3
]}

Niwa, Ayuka ^{[3
]}

Okada, Yuto ^{[3
]}

Tokeshi, Manabu ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Engn, Div Appl Chem, Kita Ku, Kita 13 Nishi 8, Sapporo, Saitama 0608628, Japan

[2] JST PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan

[3] Hokkaido Univ, Grad Sch Chem Sci & Engn, Kita Ku, Kita 13 Nishi 8, Sapporo, Hokkaido 0608628, Japan

来源：

JOURNAL OF CONTROLLED RELEASE | 2022年 / 344卷

关键词：

Lipid nanoparticles; RNA delivery; Microfluidic device; mRNA vaccine; CAS9; MESSENGER-RNA; IN-VIVO; SIRNA; SIZE; FORMULATIONS; VACCINES; DESIGN; SYSTEM; IMMUNOGENICITY; OPTIMIZATION;

D O I：

10.1016/j.jconrel.2022.02.017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In 2021, mRNA vaccines against COVID-19 were approved by the Food and Drug Administration. mRNA vaccines are important for preventing severe COVID-19 and returning to normal life. The development of RNA-delivery technology, including mRNA vaccines, has been investigated worldwide for ~30 years. Lipid nanoparticles (LNPs) are a breakthrough technology that stably delivers RNA to target organs, and RNA-loaded LNP-based nanomedicines have been studied for the development of vaccines and nanomedicines for RNA-, gene-, and cell based therapies. Recently, microfluidic devices and technologies have attracted attention for the production of LNPs, particularly RNA-loaded LNPs. Microfluidics provides many advantages for RNA-loaded LNP production, including precise LNP size controllability, high reproducibility, high-throughput optimization of LNP formulation, and continuous LNP-production processes. In this review, we summarize microfluidic-based RNA-loaded LNP production and its applications in RNA-based therapy and genome editing.

引用

页码：80 / 96

页数：17

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