Covalent Self-Assembly of Multi-Walled Carbon Nanotubes and Gold Nanoparticles for Detecting DNA Sequences of Genetically Modified Corn

被引：2

作者：

Tian, Li ^{[1
]}

Zhang, Jimei ^{[1
]}

Chen, Guangping ^{[1
]}

Li, Ying ^{[1
]}

Qi, Shibo ^{[2
]}

Li, Li ^{[2
]}

机构：

[1] Tianjin Polytech Univ, State Key Lab Hollow Fiber Membrane Mat & Proc, Sch Environm & Chem Engn, Tianjin 300387, Peoples R China

[2] Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA

来源：

ASIAN JOURNAL OF CHEMISTRY | 2013年 / 25卷 / 18期

关键词：

DNA biosensor; Multi-walled carbon nanotubes; Gold nanoparticles; Genetically modified organism; MODIFIED ORGANISMS; QUANTUM DOTS; BIOSENSORS; HYBRIDIZATION; SENSITIVITY;

D O I：

10.14233/ajchem.2013.15846

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A biosensor by a covalent self-assembly process was constructed to detect the DNA sequences of genetically modified corn based on electrochemical detection. This novel detection system consisted of the sulfhydryl-functionalized multi-walled carbon nanotubes, gold nanoparticles, DNA sequences and gold electrodes. The self-assembly processes and DNA detection were analyzed by cyclic voltammetry and different pulse voltammetry. The results showed that the DNA biosensor offered a higher selectivity and sensitivity with the detection limit of 4.03 x 10(-10) mol/L. Moreover, the biosensor has great potential to provide a general method for detecting DNA sequences of many other genetically modified organisms.

引用

页码：10535 / 10540

页数：6

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