Super-Resolution Microscopy: A Virus' Eye View of the Cell

被引：24

作者：

Grove, Joe ^{[1
]}

机构：

[1] UCL, Inst Immun & Transplantat, London NW3 2PF, England

来源：

VIRUSES-BASEL | 2014年 / 6卷 / 03期

关键词：

microscopy; super-resolution; PALM; STORM; dSTORM; tetraspanin; virus assembly; virus entry; TETRASPANIN-ENRICHED MICRODOMAINS; PHOTOACTIVATED LOCALIZATION MICROSCOPY; PAIR CORRELATION-ANALYSIS; PLASMA-MEMBRANE; ASSEMBLY SITES; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; CD81; HIV-1; MOLECULES;

D O I：

10.3390/v6031365

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and cell biology. This article provides an introduction to super-resolution imaging; in particular, localisation microscopy, and explores the application of such technologies to the study of viruses and tetraspanins, the topic of this special issue.

引用

页码：1365 / 1378

页数：14