Super-Resolution Microscopy: A Virus' Eye View of the Cell

被引:24
作者
Grove, Joe [1 ]
机构
[1] UCL, Inst Immun & Transplantat, London NW3 2PF, England
来源
VIRUSES-BASEL | 2014年 / 6卷 / 03期
关键词
microscopy; super-resolution; PALM; STORM; dSTORM; tetraspanin; virus assembly; virus entry; TETRASPANIN-ENRICHED MICRODOMAINS; PHOTOACTIVATED LOCALIZATION MICROSCOPY; PAIR CORRELATION-ANALYSIS; PLASMA-MEMBRANE; ASSEMBLY SITES; FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; CD81; HIV-1; MOLECULES;
D O I
10.3390/v6031365
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
It is difficult to observe the molecular choreography between viruses and host cell components, as they exist on a spatial scale beyond the reach of conventional microscopy. However, novel super-resolution microscopy techniques have cast aside technical limitations to reveal a nanoscale view of virus replication and cell biology. This article provides an introduction to super-resolution imaging; in particular, localisation microscopy, and explores the application of such technologies to the study of viruses and tetraspanins, the topic of this special issue.
引用
收藏
页码:1365 / 1378
页数:14
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