Flavonoid O-Diglucosyltransferase from Rice: Molecular Cloning and Characterization

被引：6
|
作者：
Kim, Bong-Gyu [1 ]
Kim, Na Yeon [1 ]
Kim, Jeong Ho [1 ]
Akimitsu, Kazuya [2 ,3 ]
Chong, Youhoon [1 ]
Ahn, Joong-Hoon [1 ]
机构：
[1] Konkuk Univ, Dept Bioscience & Biotechnol, Bio Mol Informat Ctr, Seoul 143701, South Korea
[2] Kagawa Univ, United Grad Sch, Plant Pathol Lab, Kagawa 7610795, Japan
[3] Kagawa Univ, Fac Agr, Kagawa 7610795, Japan
来源：
JOURNAL OF PLANT BIOLOGY | 2009年 / 52卷 / 01期
关键词：
Flavonoids; Flavonoid O-diglucoside; Glycosyltransferase; Oryza sativa; UGT; PLANT GLYCOSYLTRANSFERASES; EXPRESSION; GLUCOSYLTRANSFERASES; ARABIDOPSIS;
D O I：
10.1007/s12374-008-9002-x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Among more than 100 rice uridine diphosphate glycosyltransferases (UGTs), OsUGT-3 was selected as a candidate for producing flavonoid O-diglycosyltransferases based on phylogenetic analysis and molecular docking. This gene was functionally expressed in Escherichia coli. Analysis of kaempferol, luteolin, quercetin, and tricin reaction products using liquid chromatography-mass spectrometry revealed that these were diglucosylated. The glucosylation positions of kaempferol, which was the best substrate, were determined to be the 3- and 7-hydroxyl groups. This is the first flavonoid O-diglucosyltransferase described from rice.
引用
收藏
页码：41 / 48
页数：8
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