Study on the Role of Histochemical Stains in Identifying Merkel Cells in Dogs

被引:1
|
作者
Ramirez, Gustavo A. [1 ,2 ]
Espinosa de Los Monteros, Antonio [2 ]
机构
[1] Univ Lleida, Sch Agr Food Sci & Vet Med ETSEA, Dept Anim Sci, Lleida 25198, Spain
[2] Univ Las Palmas Gran Canaria, Vet Coll, Inst Anim Hlth, Unit Histol & Vet Pathol, Campus Univ Cardones, Las Palmas Gran Canaria, Spain
来源
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY | 2019年 / 302卷 / 08期
关键词
argyrophilia; CK20; Grimelius; histochemistry; Merkel cells; URANAFFIN REACTION; EPIDERMIS; SKIN; DENSITY; FETAL; LOCALIZATION; FLUORESCENCE; CHROMOGRANIN; INNERVATION; CARCINOMA;
D O I
10.1002/ar.24013
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Merkel cells (MCs) are neuroendocrine cells involved with tactile sense, growth, differentiation, and homeostasis of the skin as well as in different cutaneous diseases. Specific staining techniques are required for their identification because they are not easily visible in paraffin sections stained with hematoxylin and eosin. The present study assess the histochemical features of the MCs in dogs comparing with those described for other mammals in the literature and with the use of immunohistochemistry. A systematic study of samples from MCs-rich areas from healthy dogs was carried out by use of several histologic stains, including metachromatic staining, silver stains, methylene blue, periodic acid-Schiff stain, and osmium-based staining method. MCs were detected by the Grimelius argyrophilic stain in 86.7% of the specimens. The staining was showed as dark-brown granular cytoplasmic and consistently polarized to the basal cell cytoplasm matching with the cellular distribution of the characteristic neurosecretory granules. Some modifications in the standard staining protocol, including rinsing, silver reimpregnation, and counterstain dye, enhanced the MCs identification in stratified squamous epithelium. When compared with Cytokeratin 20-immunolabeled serial sections several MCs appeared nonstained with the argyrophilic method. These differences in MC numbers between stains were statistically significant. Other histologic stains failed to identify MCs in the specimens. The results of this study indicate that Grimelius argyrophilic stain is a suitable method for demonstration of MCs in the stratified squamous epithelium of skin and mucosa. Discussion on its utility when compared with immunohistochemistry and a review of the scientific literature is also presented. Anat Rec, 302:1458-1464, 2019. (c) 2018 Wiley Periodicals, Inc.
引用
收藏
页码:1458 / 1464
页数:7
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