Using Raman Spectroscopy and SERS for in-situ studies of rhizosphere bacteria

被引:2
|
作者
Polisetti, Sneha [1 ]
Baig, Nameera [2 ]
Bible, Amber [3 ]
Morrell-Falvey, Jennifer [3 ]
Doktycz, Mitchel [3 ]
Bohn, Paul W. [1 ,2 ]
机构
[1] Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
[2] Univ Notre Dame, Dept Chem, Notre Dame, IN 46556 USA
[3] Oak Ridge Natl Lab, BioSci Div, Oak Ridge, TN 37831 USA
来源
BIOSENSING AND NANOMEDICINE VIII | 2015年 / 9550卷
关键词
Raman spectroscopy; bacteria; SERS; auxins; pyocyanin; SURFACE-ENHANCED RAMAN; BIOFILMS;
D O I
10.1117/12.2188647
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacteria colonize plant roots to form a symbiotic relationship with the plant and can play in important role in promoting plant growth. Raman spectroscopy is a useful technique to study these bacterial systems and the chemical signals they utilize to interact with the plant. We present a Raman study of Pantoea YR343 that was isolated from the rhizosphere of Populus deltoides (Eastern Cottonwood). Pantoea sp. YR343 produce yellowish carotenoid pigment that play a role in protection against UV radiation, in the anti-oxidative pathways and in membrane fluidity. Raman spectroscopy is used to non-invasively characterize the membrane bound carotenoids. The spectra collected from a mutant strain created by knocking out the crtB gene that encodes a phytoene synthase responsible for early stage of carotenoid biosynthesis, lack the carotenoid peaks. Surface Enhanced Raman Spectroscopy is being employed to detect the plant phytoharmone indoleacetic acid that is synthesized by the bacteria. This work describes our recent progress towards utilizing Raman spectroscopy as a label free, non-destructive method of studying plant-bacteria interactions in the rhizosphere.
引用
收藏
页数:5
相关论文
共 50 条
  • [1] Raman spectroscopy analysis of DNA using the SERS method
    Varela-Fonseca, Stephanie
    Montero-Zeledon, Ernesto
    Rojas-Rojas, Laura
    Varela-Fonseca, Alejandro
    Gutierrez-Fallas, Dionisio
    TECNOLOGIA EN MARCHA, 2019, 32 (03): : 118 - 125
  • [2] In Situ Intracellular Spectroscopy with Surface Enhanced Raman Spectroscopy (SERS)-Enabled Nanopipettes
    Vitol, Elina A.
    Orynbayeva, Zulfiya
    Bouchard, Michael J.
    Azizkhan-Clifford, Jane
    Friedman, Gary
    Gogotsi, Yury
    ACS NANO, 2009, 3 (11) : 3529 - 3536
  • [3] In-situ cocrystallisation combined with Raman spectroscopy
    Kirchner, Michael T.
    Boese, Roland
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2007, 63 : S69 - S69
  • [4] In-situ cocrystallisation combined with Raman spectroscopy
    Kirchner, Michael T.
    Boese, Roland
    Blaeser, Dieter
    ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2008, 64 : C115 - C115
  • [5] Highly controlled microcrystalline silicon growth using in-situ Raman spectroscopy
    Fink, Thomas
    Muthmann, Stefan
    Meier, Matthias
    2015 IEEE 42ND PHOTOVOLTAIC SPECIALIST CONFERENCE (PVSC), 2015,
  • [6] Characterisation of fluidised bed granulation processes using in-situ Raman spectroscopy
    Walker, G. M.
    Bell, S. E. J.
    Greene, K.
    Jones, D. S.
    Andrews, G. P.
    CHEMICAL ENGINEERING SCIENCE, 2009, 64 (01) : 91 - 98
  • [7] Detection of human serum proteins using Raman and SERS spectroscopy
    Ruan, Qiuyong
    Liao, Fadian
    Lin, Juqiang
    Liu, Nenrong
    Lin, Jinyong
    Zeng, Yongyi
    Li, Ling
    Huang, Zufang
    Chen, Rong
    TWELFTH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE (PIBM 2014), 2014, 9230
  • [8] Structural Studies During Gelation of WO3 Investigated by In-situ Raman Spectroscopy
    M. Picquart
    S. Castro-Garcia
    J. Livage
    C. Julien
    E. Haro-Poniatowski
    Journal of Sol-Gel Science and Technology, 2000, 18 : 199 - 206
  • [9] In-situ curing analysis of photoreplicated objective lenses using Raman and IR spectroscopy
    Mank, A
    Verstegen, E
    Kloosterboer, H
    JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 99 (03) : 1287 - 1295
  • [10] Understanding the molecular mechanism of oxygen reduction reaction using in-situ Raman spectroscopy
    Yao-Lin, A.
    Du, Zi-Yu
    Ze, Hua-Jie
    Wang, Xiao-Ting
    Zhang, Yi
    Zhang, Hua
    Zheng, Qing-Na
    Dong, Jin-Chao
    Tian, Jing-Hua
    Li, Jian-Feng
    CURRENT OPINION IN ELECTROCHEMISTRY, 2023, 42