Optical Control of Muscle Function by Transplantation of Stem Cell-Derived Motor Neurons in Mice

被引：129

作者：

Bryson, J. Barney ^{[1
]}

Machado, Carolina Barcellos ^{[2
]}

Crossley, Martin ^{[2
]}

Stevenson, Danielle ^{[2
]}

Bros-Facer, Virginie ^{[1
]}

Burrone, Juan ^{[2
]}

Greensmith, Linda ^{[1
,3
]}

Lieberam, Ivo ^{[2
]}

机构：

[1] UCL, Inst Neurol, Sobell Dept Motor Neurosci & Movement Disord, London, England

[2] Kings Coll London, MRC, Ctr Dev Neurobiol, London WC2R 2LS, England

[3] UCL, Inst Neurol, MRC, Ctr Neuromuscular Dis, London, England

来源：

SCIENCE | 2014年 / 344卷 / 6179期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

IN-VITRO; UNITS;

D O I：

10.1126/science.1248523

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Damage to the central nervous system caused by traumatic injury or neurological disorders can lead to permanent loss of voluntary motor function and muscle paralysis. Here, we describe an approach that circumvents central motor circuit pathology to restore specific skeletal muscle function. We generated murine embryonic stem cell-derived motor neurons that express the light-sensitive ion channel channelrhodopsin-2, which we then engrafted into partially denervated branches of the sciatic nerve of adult mice. These engrafted motor neurons not only reinnervated lower hind-limb muscles but also enabled their function to be restored in a controllable manner using optogenetic stimulation. This synthesis of regenerative medicine and optogenetics may be a successful strategy to restore muscle function after traumatic injury or disease.

引用

页码：94 / 97

页数：4

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