Characterization of an excreted/secreted antigen form of 14-3-3 protein in Toxoplasma gondii tachyzoites

被引:18
作者
Assossou, O
Besson, F
Rouault, JP
Persat, F
Ferrandiz, J
Mayençon, M
Peyron, F
Picot, S
机构
[1] Univ Lyon 1, Lab Parasitol Mycol Med & Pathol Exot, Equipe Accueil, EA 3087,Fac Med, F-69373 Lyon, France
[2] UCBL, Lab Physicochim Biol, CNRS, UMR 5013, Villeurbanne, France
[3] Ctr Leon Berard, INSERM, U453, F-69373 Lyon, France
关键词
14-3-3; antibody; cycloheximide; excreted/secreted antigen; protein; Toxoplasma gondii; toxoplasmosis;
D O I
10.1016/j.femsle.2004.02.024
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The 14-3-3 protein was shown to be present into the parasitophorous vacuole of Toxoplasma gondii-infected human monocyte cells and in the excreted/secreted antigens (ESA). The ESA 14-3-3 protein migrates electrophoretically as the cytosol and the main membranous 14-3-3 isoforms. The excretion/secretion of 14-3-3 was not sensitive to cycloheximide, a protein synthesis inhibitor, even at a concentration which inhibited the production of 14-3-3 inside the tachyzoites. Recombinant 14-3-3/GST protein was used to test the presence of 14-3-3 antibodies in different human sera. A positive immunoreactivity was observed with sera corresponding to acute toxoplasmosis and a possible involvement of 14-3-3 in host immunity is discussed. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:19 / 25
页数:7
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