Compact fiber-based multi-photon endoscope working at 1700 nm

被引：53

作者：

Akhoundi, Farhad ^{[1
]}

Qin, Yukun ^{[1
]}

Peyghambarian, N. ^{[1
]}

Barton, Jennifer K. ^{[1
]}

Kieu, Khanh ^{[1
]}

机构：

[1] Univ Arizona, Coll Opt Sci, Tucson, AZ 85721 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 05期

基金：

美国国家科学基金会;

关键词：

HARMONIC-GENERATION MICROSCOPY; UNSTAINED TISSUE; LASER SYSTEM; MU-M; BRAIN; CELLS;

D O I：

10.1364/BOE.9.002326

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the design, implementation and performance analysis of a compact multi-photon endoscope based on a piezo electric scanning tube. A miniature objective lens with a long working distance and a high numerical aperture (approximate to 0.5) is designed to provide a diffraction limited spot size. Furthermore, a 1700 nm wavelength femtosecond fiber laser is used as an excitation source to overcome the scattering of biological tissues and reduce water absorption. Therefore, the novel optical system along with the unique wavelength allows us to increase the imaging depth. We demonstrate that the endoscope is capable of performing third and second harmonic generation (THG/SHG) and three-photon excitation fluorescence (3PEF) imaging over a large field of view (> 400 mu m) with high lateral resolution (2.2 mu m). The compact and lightweight probe design makes it suitable for minimally-invasive in-vivo imaging as a potential alternative to surgical biopsies. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：2326 / 2335

页数：10

共 33 条

[1] Generating femtosecond optical pulses tunable from 2 to 3 μm with a silica-based all-fiber laser system [J].