In vitro antifilarial activity of Azadirachta indica aqueous extract through reactive oxygen species enhancement

被引：6

作者：

Mukherjee, Niladri ^{[1
]}

Saini, Prasanta ^{[1
]}

Mukherjee, Suprabhat ^{[1
]}

Roy, Piiya ^{[1
]}

Babu, Santi P. Sinha ^{[1
]}

机构：

[1] Visva Bharati Univ, Ctr Adv Studies, Dept Zool, Parasitol Lab, Santini Ketan 731235, W Bengal, India

来源：

ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2014年 / 7卷 / 11期

关键词：

Azadirachta indica; Setaria cervi; Antifilarial; Reactive oxygen species; GLUTATHIONE-S-TRANSFERASE; SETARIA-CERVI; APOPTOSIS; QUANTITATION; CURCUMIN;

D O I：

10.1016/S1995-7645(14)60147-4

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Objective: To evaluate an aqueous preparation from the Azadirachta indica leaves (AEA) against Setaria cervi. (S. cervi), a model filarial parasite. Methods: In vitro efficacy of AEA was evaluated against S. cervi through estimation of relative motility value, dye exclusion test and MTT assay. Visible morphological alterations were monitored using conventional microscopic techniques in microfilariae and haematoxylin-eosin stained sections of AEA-treated adults. Results: Enhancement of reactive oxygen species in S. cervi treated with AEA was established through alteration in the activity of glutathione S-transferase, superoxide dismutase, catalase, peroxidase and level of superoxide anion and reduced glutathione. Conclusions: In vitro filaricidal activity of AEA is possibly through disturbing redox homeostasis by down-regulating and altering the level of some key antioxidants and regulatory enzymes like reduced glutathione, glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase of S. cervi.

引用

页码：841 / 848

页数：8

共 33 条

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