Chemical characteristic and bioactivity of hemicellulose-based polysaccharides isolated from Salvia miltiorrhiza

Salvia miltiorrhiza roots (SMRs), the main component of cell wall from the residual waste extraction, differ depending on the forming ways of monosaccharides. The extraction from 8% sodium hydroxide solution (H-8) was characterized by gel permeation chromatography (GPC), monosaccharide composition, Fourier transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy. The structure model of hemicellulose-based polysaccharides (HBPs) was derived by combining one-dimensional and two-dimensional NMR. Monosaccharides difference and correlation were performed by partial least square analysis (PLS). Seven H-8s exhibited optimal inhibitory activities, which varied based on different sources of Danshen. The backbone structure indicated that 4-beta-D-Xylp served as the main chain connected by 3-alpha-L-Araf or 5-alpha-L-Araf-1, 4-beta-DGalp, and beta-D-Glcp branch, as well as alpha-L-Rhap, alpha-D-GalpA and alpha-D-GlcpA fragments. The variation of HBPs in terms of the structure and bioactivity of SMRs correlatedwith different cultivation sites can be a newapproach to optimize and utilize the medical materials by chemical and biological aspects of natural macromolecules. (C) 2020 Elsevier B.V. All rights reserved.