Gas chromatography/isotope ratio mass spectrometry: Analysis of methanol, ethanol and acetic acid by direct injection of aqueous alcoholic and acetic acid samples

被引:12
作者
Ai, Guomin [1 ]
Sun, Tong [1 ]
Dong, Xiuzhu [1 ]
机构
[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
基金
中国国家自然科学基金;
关键词
SOLID-PHASE MICROEXTRACTION; ISOTOPE RATIO; FORMIC-ACID; CARBON; LIQUID; WATER; GC; C-13/C-12; SOLVENTS; BLOOD;
D O I
10.1002/rcm.6948
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
RATIONALE: Methanol, ethanol, and acetic acid are not easily extracted from aqueous samples and are susceptible to isotope fractionation in gas chromatography/isotope ratio mass spectrometry (GC/IRMS) analysis. Developing a direct dilution GC/IRMS method for aqueous samples, by adjusting the sample concentrations in common solvents to be similar to each other and using a fixed GC split ratio, is very convenient and important because any linearity effects caused by amount-dependent isotope fractionation can be avoided. METHODS: The suitability of acetonitrile and acetone solvents for the GC/IRMS analysis of pure methanol, ethanol and acetic acid, and commercial liquor and vinegar samples was evaluated using n-hexane and water as control solvents. All the solvents including water were separated from the analyte on a HP-INNOWAX column and were diverted away from the combustion interface. The influence of liquor matrix on the ethanol GC/IRMS analyses was evaluated by adding pure ethanol to liquor samples. RESULTS: Acetonitrile and acetone gave similar delta C-13 values for pure ethanol and pure acetic acid to those obtained in water and n-hexane, and also gave similar delta C-13 values of ethanol in liquor and acetic acid in white vinegar to that obtained in water. For methanol analysis, acetonitrile and refined acetone gave similar delta C-13 values to that obtained in water, but n-hexane was not a suitable solvent. In addition, isotopic fractionation caused by solvent and solute interactions was observed. CONCLUSIONS: We recommend using acetonitrile for the GC/IRMS analysis of aqueous alcoholic samples, and acetone for the analysis of aqueous acetic acid samples. This direct dilution method can provide high accurate and precise GC/IRMS analysis of the relative changes in delta C-13 values of methanol, ethanol, and acetic acid. Copyright (C) 2014 John Wiley & Sons, Ltd.
引用
收藏
页码:1674 / 1682
页数:9
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