Stage-specific quantitative changes in renal and urinary proteome during the progression and development of streptozotocin-induced diabetic nephropathy in rats

被引:8
作者
Sharma, Vikram [1 ]
Tikoo, Kulbhushan [1 ]
机构
[1] NIPER, Dept Pharmacol & Toxicol, Lab Chromatin Biol, Mohali 160062, Punjab, India
关键词
Urine; Diabetic nephropathy; Calgranulin A; Calgranulin B; MALDI-TOF/TOF; Streptozotocin; Proteomics; TYROSINE-PHOSPHATASE; GEL-ELECTROPHORESIS; GENE-EXPRESSION; HIGH-GLUCOSE; SH2; DOMAIN; E-CADHERIN; IDENTIFICATION; PROTEINS; KIDNEY; MICROALBUMINURIA;
D O I
10.1007/s11010-013-1902-5
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Diabetic nephropathy (DN) is a microvascular complication associated with diabetes causing slow deterioration of kidneys leading to end-stage renal disease. Timely intervention and diagnosis are crucial in order to ameliorate and halt the progression of DN. Current diagnosis of DN consists of urine assays for detection of microalbuminuria, which have inadequate specificity and sensitivity. Hence, there arises a need to discover stage-specific biomarkers which can aid in the early detection of DN and also in identifying the mechanisms underlying pathogenesis of DN. Therefore the present study was undertaken to identify the differentially expressed proteins in the urine and to examine the pattern of proteomic changes occurring in the rat kidneys during the course of progression of streptozotocin-induced model of DN in rats. Two-dimensional gel electrophoresis coupled to MALDI-TOF mass spectrometry was employed to identify the differentially expressed proteins under diabetic conditions. Among the identified proteins Calgranulin A and Calgranulin B appeared in the urinary proteome at the fourth week of induction of diabetes while we recorded a time-dependent decrease in the expression of major urinary protein (alpha 2u globulin) in the urine as well as kidneys of diabetic rats. Parallel monitoring of targeted proteomic changes in the renal proteome revealed an increase in histone H2B phosphorylation at serine14 along with a gradual decrease in Bcl-2 and MMP-13 expression during the course of progression and development of streptozotocin-induced DN.
引用
收藏
页码:95 / 111
页数:17
相关论文
共 60 条
[1]   Reciprocal epigenetic modification of histone H2B occurs in chromatin during apoptosis in vitro and in vivo [J].
Ajiro, K. ;
Scoltock, A. B. ;
Smith, L. K. ;
Ashasima, M. ;
Cidlowski, J. A. .
CELL DEATH AND DIFFERENTIATION, 2010, 17 (06) :984-993
[2]  
Allcock GH, 1999, J AM SOC NEPHROL, V10, P2283
[3]   CKD273, a New Proteomics Classifier Assessing CKD and Its Prognosis [J].
Argiles, Angel ;
Siwy, Justyna ;
Duranton, Flore ;
Gayrard, Nathalie ;
Dakna, Mohammed ;
Lundin, Ulrika ;
Osaba, Lourdes ;
Delles, Christian ;
Mourad, Georges ;
Weinberger, Klaus M. ;
Mischak, Harald .
PLOS ONE, 2013, 8 (05)
[4]   SOLUBLE FORMS OF THE ADHESION MOLECULE E-CADHERIN IN URINE [J].
BANKS, RE ;
PORTER, WH ;
WHELAN, P ;
SMITH, PH ;
SELBY, PJ .
JOURNAL OF CLINICAL PATHOLOGY, 1995, 48 (02) :179-180
[5]   An increased MRP8/14 expression and adhesion, but a decreased migration towards proinflammatory chemokines of type 1 diabetes monocytes [J].
Bouma, G ;
Coppens, JMC ;
Lam-Tse, WK ;
Luini, W ;
Sintnicolaas, K ;
Levering, WH ;
Sozzani, S ;
Drexhage, HA ;
Versnel, MA .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2005, 141 (03) :509-517
[6]   Effects of losartan on renal and cardiovascular outcomes in patients with type 2 diabetes and nephropathy [J].
Brenner, BM ;
Cooper, ME ;
de Zeeuw, D ;
Keane, WF ;
Mitch, WE ;
Parving, HH ;
Remuzzi, G ;
Snapinn, SM ;
Zhang, ZX ;
Shahinfar, S .
NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (12) :861-869
[7]   Differential expression of proteins in kidney, eye, aorta, and serum of diabetic and non-diabetic rats [J].
Cho, William C. ;
Yip, Tai-Tung ;
Chung, Wai-Shing ;
Leung, Albert W. ;
Cheng, Christopher H. ;
Yue, Kevin K. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 99 (01) :256-268
[8]   SHP-1 regulates Lck-induced phosphatidylinositol 3-kinase phosphorylation and activity [J].
Cuevas, B ;
Lu, YL ;
Watt, S ;
Kumar, R ;
Zhang, JY ;
Siminovitch, KA ;
Mills, GB .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (39) :27583-27589
[9]   The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells [J].
Cutillas, PR ;
Chalkley, RJ ;
Hansen, KC ;
Cramer, R ;
Norden, AGW ;
Waterfield, MD ;
Burlingame, AL ;
Unwin, RJ .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2004, 287 (03) :F353-F364
[10]  
de Moraes RV, 2007, HEAD NECK-J SCI SPEC, V30, P85