Development of a Cyclic Voltammetry-Based Method for the Detection of Antigens and Antibodies as a Novel Strategy for Syphilis Diagnosis

被引：5

作者：

Barros, Gabriel M. C. ^{[1
]}

Carvalho, Dionisio D. A. ^{[1
]}

Cruz, Agnaldo S. S. ^{[1
]}

Morais, Ellen K. L. ^{[1
]}

Sales-Moioli, Ana Isabela L. ^{[1
]}

Pinto, Talita K. B. ^{[1
,2
]}

Almeida, Melise C. D. ^{[1
]}

Sanchez-Gendriz, Ignacio ^{[1
]}

Fernandes, Felipe ^{[1
]}

Barbalho, Ingridy M. P. ^{[1
]}

Santos, Joao P. Q. ^{[1
]}

Henriques, Jorge M. O. ^{[3
]}

Teixeira, Cesar A. D. ^{[3
]}

Gil, Paulo ^{[4
]}

Gama, Lucio ^{[5
,6
]}

Miranda, Angelica E. ^{[7
]}

Coutinho, Karilany D. D. ^{[1
]}

Galvao-Lima, Leonardo J. J. ^{[1
]}

Valentim, Ricardo A. M. ^{[1
]}

机构：

[1] Fed Univ Rio Grande Norte UFRN, Hosp Univ Onofre Lopes, Lab Technol Innovat Hlth LAIS, BR-59078970 Natal, Brazil

[2] Nursing Sch Coimbra ESEnfC, Hlth Sci Res Unit Nursing UICISA E, P-3004011 Coimbra, Portugal

[3] Univ Coimbra, Univ Coimbra CISUC, Ctr Informat & Syst, Dept Informat Engn, P-3004531 Coimbra, Portugal

[4] Univ Nova Lisboa, Sch Sci & Technol, Dept Elect & Comp Engn, P-1099085 Lisbon, Portugal

[5] Johns Hopkins Univ, Sch Med, Dept Mol & Comparat Biol, Baltimore, MD 21218 USA

[6] NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

[7] Univ Fed Espirito Santo, Postgrad Program Infect Dis, BR-29075910 Vitoria, Brazil

来源：

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2022年 / 19卷 / 23期

关键词：

syphilis; Treponema pallidum; electrodes; point-of-care method; screening strategy; PRINTED CARBON ELECTRODES;

D O I：

10.3390/ijerph192316206

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The improvement of laboratory diagnosis is a critical step for the reduction of syphilis cases around the world. In this paper, we present the development of an impedance-based method for detecting T. pallidum antigens and antibodies as an auxiliary tool for syphilis laboratory diagnosis. We evaluate the voltammetric signal obtained after incubation in carbon or gold nanoparticle-modified carbon electrodes in the presence or absence of Poly-L-Lysine. Our results indicate that the signal obtained from the electrodes was sufficient to distinguish between infected and non-infected samples immediately (T0 ') or 15 min (T15 ') after incubation, indicating its potential use as a point-of-care method as a screening strategy.

引用

页数：10

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