Update on proteomic studies of formalin-fixed paraffin-embedded tissues

被引：17
作者：
Giusti, Laura [1 ]
Angeloni, Cristina [1 ]
Lucacchini, Antonio [2 ]
机构：
[1] Univ Camerino, Sch Pharm, Camerino, Italy
[2] Univ Pisa, Dept Clin & Expt Med, Pisa, Italy
来源：
EXPERT REVIEW OF PROTEOMICS | 2019年 / 16卷 / 06期
关键词：
Biomarkers; cancer; formalin-fixed paraffin-embedded tissues; fresh; frozen tissues; imaging mass spectrometry; mass spectrometry; proteomics; MASS-SPECTROMETRY ANALYSIS; FFPE TISSUE; PREANALYTICAL FACTORS; CELL CARCINOMA; PROTEIN; EXTRACTION; DEPARAFFINIZATION; MICROPROTEOMICS; CLASSIFICATION; IDENTIFICATION;
D O I：
10.1080/14789450.2019.1615452
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Introduction: This review is an update on recent progress in proteomic studies of formalin-fixed paraffin-embedded (FFPE) tissues, which open the opportunity to investigate diseases and research potential biomarkers, particularly when availability of fresh/frozen tissues is low. Areas covered: We described improvement of existing protocols or the new ones regarding deparaffinization and protein extraction of FFPE samples published from 2014 to today. Moreover, the growing interest to use FFPE tissues for mass spectrometry imaging approach is presented together with the search of post-translational modifications. Expert opinion: In the last few years, the number of papers using FFPE tissues in proteomic analysis is growing. The interest to apply proteomic analysis to FFPE tissues lies in the easy accessibility of a great number of samples from archives. Nevertheless, standardization in the approach among the different researchers is not achieved, making essentially incomparable the results obtained. This limit should be overcome.
引用
收藏
页码：513 / 520
页数：8
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