Inactivation of putative PKS genes can double geldanamycin yield in Streptomyces hygroscopicus 17997

被引：2

作者：

Li, Y. H. ^{[1
,2
]}

Li, M. ^{[1
]}

He, W. Q. ^{[2
]}

Wang, Y. G. ^{[2
]}

Shao, R. G. ^{[2
]}

机构：

[1] Xinxiang Med Univ, Henan Cultivating Key Lab Hereditary Dis Targeted, Sch Life Sci & Technol, Xinxiang, Peoples R China

[2] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Biotechnol, Beijing 100730, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 02期

关键词：

Geldanamycin; Inactivation; PKS; Hsp90; inhibitor; BIOSYNTHESIS; IDENTIFICATION; STREPTOMYCES-HYGROSCOPICUS-17997;

D O I：

10.4238/2013.June.21.3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The putative polyketide biosynthesis (PKS) genes cos10 and pg10 were inactivated by insertion of a kanamycin-resistance gene into the genome of the geldanamycin-producing strain, Streptomyces hygroscopicus 17997. The resultant inactivation were confirmed by PCR analysis. The abilities of the PKS gene inactivation strains to produce geldanamycin were compared with the natural geldanamycin-producing strain, S. hygroscopicus 17997. The cos10-inactivated strain exhibited an unchanged ability to produce geldanamycin, but the pg10-inactivated strain can produce twice the yield of the natural strain when grown under the same conditions. We propose that there is a sub-PKS pathway in the geldanamycin-producing strain, S. hygroscopicus 17997.

引用

页码：2076 / 2085

页数：10

共 19 条

[1] GELDANAMYCIN, A NEW ANTIBIOTIC [J].