Development of Co-hemin MOF/chitosan composite based biosensor for rapid detection of lactose

被引:30
作者
Choi, Han Suk [1 ]
Yang, Xiaoguang [1 ]
Liu, Guicheng [2 ]
Kim, Dong Sup [1 ]
Yang, Ji Hyun [1 ]
Lee, Ju Hun [1 ]
Han, Sung Ok [3 ]
Lee, Jinyoung [4 ]
Kim, Seung Wook [1 ]
机构
[1] Korea Univ, Dept Chem & Biol Engn, 145 Anam Ro, Seoul 02841, South Korea
[2] Dongguk Univ, Dept Phys, 30,Pildong Ro 1 Gil, Seoul 04620, South Korea
[3] Korea Univ, Dept Biotechnol, 145 Anam Ro, Seoul 02841, South Korea
[4] Sangmyung Univ, Gyedang Coll Gen Educ, 31 Sangmyeongdae Gil, Cheonan Si, Chungcheongnam, South Korea
基金
新加坡国家研究基金会;
关键词
Electrochemical b osensor; Co-hemin; Metal organic frameworks; Cellobiose dehydrogenase; Lactose; CELLOBIOSE DEHYDROGENASE; 3RD-GENERATION BIOSENSOR; CARBON NANOTUBES; NANOPARTICLES; GOLD;
D O I
10.1016/j.jtice.2020.07.021
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
An electrochemical biosensor containing Co-hemin metal organic frameworks (Co-hemin MOF)/chitosan composite and cellobiose dehydrogenase (CDH) was developed for lactose dectection. The formation of the Co-hemin MOF was verified by scanning electron microscopy, Fourier transform infrared spectroscopy, UV-vis absorption spectroscopy and electrochemical techniques. The Co-hemin MOF/chitosan composite exhibited outstanding electrochemical performance on lactose detection with high sensitivity (102.3 mu A mM(-1) cm(-2)) and fast response (5 s). More importantly, the lower determination limit of 4 mM and the broad linear ranges of 10 to 100 mM were obtained to detect lactose. With broad linear ranges, the feasibility of dairy application of the biosensor based on Co-hemin MOF electrode was demonstrated in this study. (C) 2020 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 7
页数:7
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