Tellurium-based mass cytometry barcode for live and fixed cells

被引：24

作者：

Willis, Lisa M. ^{[1
]}

Park, Hanuel ^{[1
]}

Watson, Michael W. L. ^{[2
]}

Majonis, Daniel ^{[3
]}

Watson, Jessica L. ^{[3
]}

Nitz, Mark ^{[1
]}

机构：

[1] Univ Toronto, Dept Chem, 80 St George St, Toronto, ON M5S 3H6, Canada

[2] Fluidigm Corp, 7000 Shoreline Court, San Francisco, CA 94080 USA

[3] Fluidigm Canada Inc, 1380 Rodick Rd, Markham, ON L3R 4G5, Canada

来源：

CYTOMETRY PART A | 2018年 / 93A卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Key terms mass cytometry; tellurium; tellurophene; barcode; Callisto(TM); mass tag; CyTOF-2(TM); Helios(TM) mRNA-Seq; toxicity; T-CELLS; ACTIVATION; IMMUNITY; REAGENT; INNATE; BLOOD; CYCLE;

D O I：

10.1002/cyto.a.23495

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mass cytometry is a revolutionary technology that allows for the simultaneous quantification of >40 different biomarkers with cellular resolution. The biomarkers are detected using metal-labeled antibodies as well as small-molecule probes of cell size, viability, and biochemical status. Barcoding is an important component of sample preparation because it reduces processing time, eliminates sample-to-sample variation, discriminates cell doublets, reduces the amount of antibody needed, and conserves sample. We developed a thiol-reactive tellurium-based barcode, TeMal. TeMal is nontoxic at working concentrations, compatible with metal-labeled antibodies, and can readily be applied to live or fixed cells, making it advantageous and complementary compared to existing barcoding reagents. We have demonstrated the utility of TeMal by barcoding microscale samples in situ to facilitate analysis of cells from an automated cell culture system using mass cytometry.

引用

页码：685 / 694

页数：10

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