Detection of Salmonella spp. in retail meat products: a comparison between a discontinued commercial kit and a laboratory-developed screening method

被引：1

作者：

Shushe, O. ^{[1
]}

Wroblewski, D. ^{[2
]}

MacGowan, C. E. ^{[2
]}

Passaretti, T. ^{[2
]}

Musser, K. ^{[2
]}

Mingle, L. ^{[2
]}

机构：

[1] Colgate Univ, Hamilton, NY 13346 USA

[2] Wadsworth Ctr NYS Dept Hlth, Albany, NY USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2019年 / 69卷 / 02期

关键词：

antimicrobial resistance; public health; real-time PCR; Salmonella spp; Taqman; SELECTIVE ENRICHMENT;

D O I：

10.1111/lam.13185

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The National Antimicrobial Resistance Monitoring System for Enteric Bacteria retail food surveillance programme screens retail meat samples for the presence of Salmonella spp. to track antimicrobial resistance in food. In this study, a laboratory developed real-time PCR assay that detects Salmonella spp. was evaluated as a screening method to replace the discountinued 3M TECRA kit. The 3M TECRA kit was a commercially available, visual immunoassay used to screen food samples for the presence of Salmonella spp. This kit was discontinued in September 2016 by the manufacturer and an alternative screening method was needed to replace the discontinued TECRA kit. Salmonella spp. is detected by the real-time PCR assay earlier in the screening process than by the TECRA kit. Salmonella spp. can also be reliably isolated from the enrichment broth earlier in the protocol. Additionally, cost analysis shows that the real-time PCR assay saves $2 center dot 50 per sample. New York State Department of Health currently uses this real-time PCR assay as a screening method for the presence of Salmonella spp. in retail meat samples. The assay allows for continued monitoring of antimicrobial resistance in Salmonella spp., while providing a cost savings and a decrease in turnaround time. Significance and Impact of the Study The National Antimicrobial Resistance Monitoring System for Enteric Bacteria (NARMS) tracks antimicrobial susceptibility of enteric bacteria in people, food and animals (). The New York State Department of Health (NYSDOH) became a NARMS retail food surveillance (RFS) site in 2003. The NARMS-RFS programme screens retail meat samples from grocery stores in the United States for the presence of Salmonella spp. and other enteric pathogens to monitor the prevalence of antimicrobial resistance among these pathogens. The NYSDOH developed a rapid and cost-effective real-PCR assay to screen for Salmonella spp. in retail meat products.

引用

页码：116 / 120

页数：5