Sesquiterpenoids, phenolic and lignan glycosides from the roots and rhizomes of Clematis hexapetala Pall. and their bioactivities

被引:12
作者
Cai, Lu [1 ]
Shao, Si-Yuan [1 ]
Han, Shao-Wei [1 ]
Li, Shuai [1 ]
机构
[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, State Key Lab Bioact Subst & Funct Nat Med, Beijing 100050, Peoples R China
关键词
Clematis hexapetala Pall; Guaianolide sesquiterpenoids; Phenolic glycosides; Lignan glycosides; Anti-inflammatory activity; PTP1B inhibition; TRITERPENE SAPONINS; BIOSYNTHESIS; CONSTITUENTS; ANTIOXIDANT; TERPENOIDS; MTPA;
D O I
10.1016/j.bioorg.2020.104312
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Approximately 17 compounds were isolated from a 60% EtOH aqueous extract of the roots and rhizomes of Clematis hexapetala Pall., including three new guaianolide sesquiterpenoids with 5/7/5-fused rings and 3Sconfiguration (1-3), five new prenylated tetra-substituted phenolic glycosides (4-8) with 6/6-fused 9H-benzopyran skeleton (5) and 6/7-fused 7,10-dihydro-benzoxepin skeleton (6-8), one new isoferulyl glucoside (9), two new furofuran lignan diglucosides (10-11), and six known compounds. The chemical structures of the new compounds were elucidated via spectroscopic data and electronic circular dichroism (ECD) analyses in combination with a modified Mosher's method. The possible biosynthetic relationships of prenylated tetra-substituted phenols were postulated. In the in vitro assays, compound 16 exhibited moderate TNF-alpha secretion inhibitory activity with IC50 value of 3.419 mu M. Compounds 14-16 displayed potent PTP1B enzymatic inhibitory activities with inhibition ratios of 48.30-86.00%. And compound 16 showed significant PTP1B enzymatic inhibition with IC50 value of 4.623 mu M.
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页数:11
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