Detachment and flow cytometric quantification of seagrass-associated bacteria

被引：5

作者：

Trevathan-Tackett, Stacey ^{[1
]}

Macreadie, Peter ^{[1
]}

Ralph, Peter ^{[1
]}

Seymour, Justin ^{[1
]}

机构：

[1] Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Sydney, NSW 2007, Australia

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 102卷

关键词：

Bacterial detachment; Flow cytometry; Seagrass-associated bacteria; Enumeration; Microbe; Seagrass; VASCULAR PLANT DETRITUS; AQABA JORDAN; DECOMPOSITION; SEDIMENTS; LITTER; CARBON; GROWTH; PARTICLES; MEADOWS; ROOTS;

D O I：

10.1016/j.mimet.2014.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new protocol was developed to detach bacteria from seagrass tissue and subsequently enumerate cells using flow cytometry (FCM). A method involving addition of the surfactant Tween 80 and vortexing resulted in maximum detachment efficiency of seagrass attached bacteria, providing a robust protocol for precisely enumerating seagrass-associated bacteria with FCM. Using this approach we detected cell concentrations between 2.0 x 10(5) and 8.0 x 10(6) cells mg(-1) DW tissue. 2014 Elsevier B.V. All rights reserved.

引用

页码：23 / 25

页数：3

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