By-products of Scyliorhinus canicula, Prionace glauca and Raja clavata: A valuable source of predominantly 6S sulfated chondroitin sulfate

被引：41

作者：

Novoa-Carballal, Ramon ^{[1
,2
]}

Perez-Martin, Ricardo ^{[3
]}

Blanco, Maria ^{[3
]}

Sotelo, Carmen G. ^{[3
]}

Fassini, Dario ^{[1
,2
]}

Nunes, Claudia ^{[4
,5
]}

Coimbra, Manuel A. ^{[5
]}

Silva, Tiago H. ^{[1
,2
]}

Reis, Rui L. ^{[1
,2
]}

Antonio Vazquez, Jose ^{[6
]}

机构：

[1] Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Headquarters European Inst Excellence Tissue Engn, AvePk, P-4805017 Barco, Guimaraes, Portugal

[2] ICVS 3Bs PT Govt Associate Lab, Braga, Portugal

[3] CSIC, Marine Res Inst IIM, Grp Food Biochem, R Eduardo Cabello 6, Vigo 36208, Pontevedra, Spain

[4] Univ Aveiro, Dept Quim, CICECO, Campus Santiago, P-3810193 Aveiro, Portugal

[5] Univ Aveiro, Dept Quim, QOPNA, Campus Santiago, P-3810193 Aveiro, Portugal

[6] CSIC, Marine Res Inst IIM, Grp Recycling & Valorisat Waste Mat REVAL, R Eduardo Cabello 6, Vigo 36208, Pontevedra, Spain

来源：

CARBOHYDRATE POLYMERS | 2017年 / 157卷

关键词：

Chondroitin sulfate; Elasmobranch; Scyliorhinus canicula; Prionace glauca; Raja clavata; Sulfation position; SIZE-EXCLUSION CHROMATOGRAPHY; HYALURONIC-ACID; URONIC-ACIDS; CARTILAGE; GLYCOSAMINOGLYCANS; CHITOSANS; PROTEINS;

D O I：

10.1016/j.carbpol.2016.09.050

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Chondroitin sulfate (CS) was isolated from Scyliorhinus canicula (fin, head and skeleton), Prionace glauca (head), and Raja clavata (skeleton) by-products from fish processing industry using environmentally friendly processes. The molecular weight was determined by gel permeation chromatography and the sugar composition and sulfation position by NMR and SAX-HPLC after enzymatic digestion. The CSs showed a prevalent 6S GaINAc sulfation for the 3 species (4S/6S ratio lower than 1). A higher 6S sulfation was observed for P. glauca head and R. clavata skeleton (4S/6S ratio below 0.20) than for S. canicula (4S/6S ratio ca. 0.6). The existence of CS samples with such low 4S/6S ratio has only been observed before in a rare species of shark (Mitsukutina owatoni, globin shark). The good extraction yields achieved make S. canicula, P. glauca and R. clavata fish industry by-products a useful source of 6-sulfated chondroitin sulfate. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：31 / 37

页数：7

共 32 条

[31]

Yamada Shuhei, 2011, Commun Integr Biol, V4, P150, DOI 10.4161/cib.4.2.14547

[32]

YAMAGATA T, 1968, J BIOL CHEM, V243, P1523

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