Architectural requirements for optimal activation by tandem CRP molecules at a class ICRP-dependent promoter

被引：25

作者：

Tebbutt, J ^{[1
]}

Rhodius, VA ^{[1
]}

Webster, CL ^{[1
]}

Busby, SJW ^{[1
]}

机构：

[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 210卷 / 01期

关键词：

transcription activation; cAMP receptor protein; RNA polymerase alpha subunit; CRP-dependent promoter;

D O I：

10.1111/j.1574-6968.2002.tb11159.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Escherichia coli cyclic AMP receptor protein (CRP) activates transcription at target promoters by interacting with the C-terminal domain of the RNA polymerase a subunit. We have constructed a set of promoters carrying tandem DNA sites for CRP with one site centred at position -61.5 and the other site located at different upstream positions. Optimal CRP-dependent activation of transcription is observed when the upstream DNA site for CRP is located at position -93.5 or at position -103.5. Evidence is presented to suggest that activation by the upstream-bound CRP molecule is due to interaction with the C-terminal domain of the RNA polymerase (X subunit. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：55 / 60

页数：6

共 10 条

[1] Transcription activation at promoters carrying tandem DNA sites for the Escherichia coli cyclic AMP receptor protein:: Organisation of the RNA polymerase α subunits [J].

Belyaeva, TA ;

Rhodius, VA ;

Webster, CL ;

Busby, SJW .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (04) :789-804

[2] Transcription activation by catabolite activator protein (CAP) [J].

Busby, S ;

Ebright, RH .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 293 (02) :199-213

[3] TRANSCRIPTION ACTIVATION BY THE ESCHERICHIA-COLI CYCLIC-AMP RECEPTOR PROTEIN - RECEPTORS BOUND IN TANDEM AT PROMOTERS CAN INTERACT SYNERGISTICALLY [J].

BUSBY, S ;

WEST, D ;

LAWES, M ;

WEBSTER, C ;

ISHIHAMA, A ;

KOLB, A .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 241 (03) :341-352

[4] ROLE OF GLUTAMIC ACID-181 IN DNA-SEQUENCE RECOGNITION BY THE CATABOLITE GENE ACTIVATOR PROTEIN (CAP) OF ESCHERICHIA-COLI - ALTERED DNA-SEQUENCE-RECOGNITION PROPERTIES OF [VAL181]CAP AND [LEU181]CAP [J].

EBRIGHT, RH ;

KOLB, A ;

BUC, H ;

KUNKEL, TA ;

KRAKOW, JS ;

BECKWITH, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (17) :6083-6087

[5]

GUNASEKERA A, 1992, J BIOL CHEM, V267, P14713

[6] SYNERGISTIC ACTIVATION OF TRANSCRIPTION BY ESCHERICHIA-COLI CAMP RECEPTOR PROTEIN [J].

JOUNG, JK ;

LE, LU ;

HOCHSCHILD, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :3083-3087

[7] A genetic method for dissecting the mechanism of transcriptional activator synergy by identical activators [J].

Langdon, RC ;

Hochschild, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12673-12678

[8] Interactions between the Escherichia coli cAMP receptor protein and the C-terminal domain of the α subunit of RNA polymerase at Class I promoters [J].

Law, EC ;

Savery, NJ ;

Busby, SJW .

BIOCHEMICAL JOURNAL, 1999, 337 :415-423

[9]

SAVERY N, 2002, IN PRESS J BACTERIOL, V184

[10] Transcription activation at class II CRP-dependent promoters:: identification of determinants in the C-terminal domain of the RNA polymerase α subunit [J].

Savery, NJ ;

Lloyd, GS ;

Kainz, M ;

Gaal, T ;

Ross, W ;

Ebright, RH ;

Gourse, RL ;

Busby, SJW .

EMBO JOURNAL, 1998, 17 (12) :3439-3447

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