Synthesis of large-ring cyclodextrin from tapioca starch by amylomaltase and complex formation with vitamin E acetate for solubility enhancement

被引：32

作者：

Kuttiyawong, K. ^{[1
]}

Saehu, S. ^{[2
]}

Ito, K. ^{[3
]}

Pongsawasdi, P. ^{[2
]}

机构：

[1] Kasetsart Univ, Fac Liberal Arts & Sci, Nakhon Pathom 73140, Thailand

[2] Chulalongkorn Univ, Dept Biochem, Starch & Cyclodextrin Res Unit, Fac Sci, Bangkok 10330, Thailand

[3] Osaka City Univ, Grad Sch Sci, Lab Enzyme Chem, Osaka 5588585, Japan

来源：

PROCESS BIOCHEMISTRY | 2015年 / 50卷 / 12期

关键词：

Large-ring cyclodextrin; Amylomaltase; Tapioca starch; Vitamin E acetate; Solubility enhancement; X-RAY-STRUCTURE; INCLUSION COMPLEXES; BETA-CYCLODEXTRIN; THERMUS-AQUATICUS; ALPHA-TOCOPHEROL; CYCLOAMYLOSE; 4-ALPHA-GLUCANOTRANSFERASE; EXPRESSION; AMYLOSE;

D O I：

10.1016/j.procbio.2015.10.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant amylomaltase from. Corynebacterium glutamicum (CgAM) was used to synthesize large-ring cyclodextrins (LR-CDs) from tapioca starch. A mixture of LR-CDs (CD22-CD54) was obtained from both raw and soluble tapioca starch, but with different ratio of main products (CD25-CD30). Almost threefold higher amount of products was formed indicating the preference for raw tapioca starch. The optimal condition was to incubate 1.0% (w/v) starch with 0.05 U/ml CgAM in buffer pH 6.0 at 30 degrees C for 18 h. Total LR-CDs yield was 60.5 mg/g starch. LR-CDs were resistant to hydrolysis by Rhizopus sp. glucoamylase but completely hydrolyzed by CgAM at high enzyme concentration. Solid inclusion complex between LR-CDs mixture and vitamin E acetate was formed, as analyzed by FTIR. For complex preparation, the 1:10 mol ratio of LR-CDs to vitamin E was chosen because maximum amount of the vitamin was bound to LR-CDs and high complex yield was obtained. Aqueous solubility of vitamin E acetate in complex form increased 800-fold while the antioxidant activity decreased about 30%. This is the first work to demonstrate the use of tapioca starch in LR-CDs synthesis by CgAM and the complex formation between LR-CDs and vitamin E acetate as solubility enhancement process of vitamin. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：2168 / 2176

页数：9

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