Proteolytic activity of Lactobacillus strains isolated from Mongolian traditional dairy products: A multiparametric analysis

被引:28
|
作者
Raveschot, Cyril [1 ,2 ]
Cudennec, Benoit [1 ]
Deracinois, Barbara [1 ]
Fremont, Marc [2 ]
Vaeremans, Maxime [2 ]
Dugersuren, Jamyan [3 ]
Demberel, Shirchin [3 ]
Drider, Djamel [1 ]
Dhulster, Pascal [1 ]
Coutte, Francois [1 ]
Flahaut, Christophe [1 ]
机构
[1] Univ Littoral Cote dOpale, Univ dArtois, Inst Charles Viollette, Univ Lille,INRA,ISA,EA 7394 ICV, F-59000 Lille, France
[2] VF Biosci, Parc Eurasante, F-59120 Loos Lez Lille, France
[3] Mongolian Vet Inst, Probiot Res Ctr, Ulan Bator 17024, Mongolia
关键词
Cell envelope associated peptidases; Fermented milk; Lactobacillus; Peptides; LACTIC-ACID BACTERIA; BIOACTIVE PEPTIDES; FERMENTED MILK; HELVETICUS; DIVERSITY; PROTEINASES; BULGARICUS; ENZYMES; METABOLISM; CULTURES;
D O I
10.1016/j.foodchem.2019.125415
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The aim of our study was to characterize the proteolytic activity of 170 Lactobacillus strains isolated from traditional Mongolian dairy products (yogurt and fermented milk), and to investigate their capacity to generate bioactive peptides during milk fermentation. All isolates were screened for proteolytic activity using skim milk agar-well diffusion test. Fifteen strains (9 Lactobacillus helveticus and 6 Lactobacillus delbrueckii subsp. bulgaricus) were then selected and further evaluated using an original strategy based on multiparametric analysis, taking into account growth rate, acidification capacity, proteolytic activity, cell envelope associated peptidase (CEP) profile and LC-MS/ MS analysis of peptides. All parameters were analyzed using principal component analysis (PCA). Results showed that strain growth and acidification correlate with peptide production and that Mongolian L. helveticus strains differ from Western strains in terms of CEP distribution. The PCA revealed that CEP profiles are major determinants of beta-casein hydrolysis patterns. Strains with distinctive proteolytic activities were identified.
引用
收藏
页数:9
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