Regeneration of eggplant (Solanum melongena L.) from root explants

被引:42
作者
Franklin, G
Sheeba, CJ
Sita, GL
机构
[1] Indian Inst Sci, Dept Microbiol & Cell Biol, Bangalore 560012, Karnataka, India
[2] Loyola Coll, Entomol Res Inst, Madras 600034, Tamil Nadu, India
关键词
indirect organogenesis; Soilrite (R); Solanum; thidiazuron;
D O I
10.1079/IVP2003491
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Eggplant (Solanum melongena L.) was efficiently regenerated from cultured roots of 15-d-old seedlings on Murashige and Skoog (MS) medium containing 0.45 muM thidiazuron and 13.3 muM 6-benzyladenine. Within 28d of culture initiation, induction of organogenic calluses and subsequent differentiation into shoot buds were observed. Shoot buds upon subculture to MS basal medium elongated into healthy shoots. Excised shoots (2-4 cm) were rooted on Soilrite(R) irrigated with water either in vitro or in vivo. Plants with well-developed root systems were established under field conditions after hardening in the glasshouse, where they developed into flowering plants and produced mature fruits with viable seeds.
引用
收藏
页码:188 / 191
页数:4
相关论文
共 17 条
[11]  
MORTON L, 1991, PLANT CELL REP, V10, P235
[12]   LOW SUGAR AND OSMOTIC REQUIREMENTS FOR SHOOT REGENERATION FROM LEAF PIECES OF SOLANUM-MELONGENA L [J].
MUKHERJEE, SK ;
RATHINASABAPATHI, B ;
GUPTA, N .
PLANT CELL TISSUE AND ORGAN CULTURE, 1991, 25 (01) :13-16
[13]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497
[14]  
RAO PVL, 1991, PLANT CELL REP, V10, P7, DOI 10.1007/BF00233023
[15]   IMPROVED CULTURE CONDITIONS FOR SOMATIC EMBRYOGENESIS USING AN ASEPTIC VENTILATIVE FILTER IN EGGPLANT (SOLANUM-MELONGENA L) [J].
SAITO, T ;
NISHIMURA, S .
PLANT SCIENCE, 1994, 102 (02) :205-211
[16]   GENOTYPE, EXPLANT AND POSITION EFFECTS ON ORGANOGENESIS AND SOMATIC EMBRYOGENESIS IN EGGPLANT (SOLANUM-MELONGENA L) [J].
SHARMA, P ;
RAJAM, MV .
JOURNAL OF EXPERIMENTAL BOTANY, 1995, 46 (282) :135-141
[17]   CULTURAL-BEHAVIOR OF PROTOPLASTS FROM DIFFERENT ORGANS OF EGGPLANT (SOLANUM-MELONGENA-L), AND PLANT-REGENERATION [J].
SIHACHAKR, D ;
DUCREUX, G .
PLANT CELL TISSUE AND ORGAN CULTURE, 1987, 11 (03) :179-188