Circular RNA 0000096 affects cell growth and migration in gastric cancer

被引：227

作者：

Li, Peifei ^{[1
,3
]}

Chen, Huilin ^{[2
]}

Chen, Shengcan ^{[1
]}

Mo, Xiaoyan ^{[1
]}

Li, Tianwen ^{[1
]}

Xiao, Bingxiu ^{[1
]}

Yu, Rui ^{[1
]}

Guo, Junming ^{[1
]}

机构：

[1] Ningbo Univ, Dept Biochem & Mol Biol, Zhejiang Key Lab Pathophysiol, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Coll Hlth Sci, Dept Human Body Funct, Ningbo 315010, Zhejiang, Peoples R China

[3] Ningbo First Hosp, Dept Gastroenterol, 59 Liuting St, Ningbo 315010, Zhejiang, Peoples R China

来源：

BRITISH JOURNAL OF CANCER | 2017年 / 116卷 / 05期

关键词：

cell growth; gastric cancer; molecular carcinogenesis; RNA expression; BREAST-CANCER; MESSENGER-RNAS; GENE; EXON; EXPRESSION; TRANSCRIPTS; DATABASE; PTEN;

D O I：

10.1038/bjc.2016.451

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Circular RNAs (circRNAs) are a class of non-coding RNAs broadly expressed in cells of various species. Their role in cancers, especially in gastric cancer, is poorly understood. Methods: Circular RNA 0000096 (hsa_circ_0000096) levels in 101 paired gastric cancer tissues and adjacent non-tumorous tissues from patients with gastric cancer were detected by real-time quantitative reverse transcription-polymerase chain reaction. A receiver operating characteristic curve was generated to evaluate the diagnostic value of hsa_circ_0000096. RNA interference was used to manipulate the expression of hsa_circ_0000096. Its biological effects were evaluated by flow cytometry, real-time cell analysis, a wound scratch assay, western blot analysis and xenograft models. Results: Hsa_circ_0000096 was found to be significantly downregulated in gastric cancer tissues and gastric cancer cell lines compared with paired adjacent non-tumorous tissues and normal gastric epithelial cells (P<0.001). Moreover, knockdown of hsa_circ_0000096 significantly inhibited cell proliferation and migration in vitro and in vivo. The results of both immunohistochemical and western blot analyses showed that the protein levels of cyclin D1, cyclin-dependent kinase 6 (CDK6), matrix metalloproteinase-2 and MMP-9 were significantly reduced in vitro and in vivo. A gastric cancer xenograft nude mouse model indicated that Ki67 and VEGF were reduced in a dose-dependent manner following knockdown of hsa_circ_0000096. However, the expression of E-cadherin increased. Conclusions: Hsa_circ_0000096 may be used as a potential novel biomarker for gastric cancer. It affects gastric cancer cell growth and migration by regulating cyclin D1, CDK6, MMP-2 and MMP-9.

引用

页码：626 / 633

页数：8

共 34 条

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