Microfluidic devices for measuring gene network dynamics in single cells

被引：189

作者：

Bennett, Matthew R. ^{[1
,2
]}

Hasty, Jeff ^{[3
,4
]}

机构：

[1] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA

[2] Rice Univ, Inst Biosci & Bioengn, Houston, TX 77005 USA

[3] Univ Calif San Diego, Dept Mol Biol, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Dept Bioengn, BioCircuits Inst, La Jolla, CA 92093 USA

来源：

NATURE REVIEWS GENETICS | 2009年 / 10卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ON-A-CHIP; SACCHAROMYCES-CEREVISIAE; FLUORESCENT PROTEINS; SYSTEMS BIOLOGY; YEAST-CELLS; QUANTITATIVE-ANALYSIS; BACTERIAL CHEMOTAXIS; MESSENGER-RNA; EXPRESSION; GRADIENTS;

D O I：

10.1038/nrg2625

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The dynamics governing gene regulation have an important role in determining the phenotype of a cell or organism. From processing extracellular signals to generating internal rhythms, gene networks are central to many time-dependent cellular processes. Recent technological advances now make it possible to track the dynamics of gene networks in single cells under various environmental conditions using microfluidic 'lab-on-a-chip' devices, and researchers are using these new techniques to analyse cellular dynamics and discover regulatory mechanisms. These technologies are expected to yield novel insights and allow the construction of mathematical models that more accurately describe the complex dynamics of gene regulation.

引用

页码：628 / 638

页数：11

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